川芎-天麻不同提取物对大鼠血瘀型偏头痛药效学差异研究  

Pharmacodynamic Differences of Different Extraction of Rhizoma Ligusticum Chuanxiong and Gastrodia Elata in Migraine Rats with Blood Stasis

作　　者：刘明平[1] 黄曼婷[1] 毛禹康 黄志云[1] 周赛妮 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《辽宁中医药大学学报》2016年第2期5-7,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81274107)

摘　　要：目的:比较川芎-天麻药对不同提取物对血瘀证偏头痛大鼠的药效学差异。方法:雄性SD大鼠随机分为9组,即为正常对照组、模型对照组、阳性药盐酸氟桂利嗪(西比灵)组、川芎-天麻(4∶1,)水提物组、川芎-天麻(1∶1)水提物组、川芎-天麻(1∶4)水提物组、川芎-天麻(4∶1)醇提物组、川芎-天麻(1∶1)醇提物组、川芎-天麻(1∶4)醇提物组。大鼠冰水浴后皮下注射盐酸肾上腺素(0.8 mg/kg)和硝酸甘油(10 mg/kg)复制血瘀证偏头痛模型,比色法测定大鼠血清一氧化氮合酶(NOS)活力与一氧化氮(NO)水平,Elisa法测定血清降钙素基因相关肽(CGRP)、内皮素(ET)、血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)水平并计算NO/ET。结果:模型组血清CGRP水平、NOS活力、NO、TXB2含量及NO/ET比值升高,6-keto-PGF1α含量降低,与正常组比较差异有显著性(P<0.05);各比例提取物组间比较,各比例组醇提物NO水平及NO/ET比值均低于同比例水提物(P<0.05),6-Keto-PGF1α(4∶1组)水平高于同比例水提物(P<0.05)。结论:川芎-天麻不同提取物对血瘀型偏头痛均有一定疗效,不同提取物组间疗效有差异,综合多项指标考虑,醇提物疗效优于水提物。Objective：To study the pharmacodynamic differences of migraine rat models with blood stasis treated with different proportion of Rhizoma Ligusticum chuanxiong and Gastrodia Elata（4∶1,1∶1,1 ∶ 4）extracted with water and 70% alcohol. Methods：SD rats were randomly divided into 9 groups：normal group,model group,Flunarizine group and different propotion of Rhizoma Ligusticum chuanxiong and Gastrodia Elata（4∶1）,（1∶1）,（1∶4）water and alcohol extract group. Adrenaline（s.c. 0.8 mg/kg）and ice water bath were used to establish acute blood stasis model,migraine models were copied by glyceryltrinitrate（s.c. 10 mg/kg）. Then determined the content of CGRP,NOS,NO,ET,TXB2,6-ketoPGF1α in rat serum. Results：In model group,The levels of CGRP,NOS,NO,TXB2 and the ratio of NO/ET raised（P0.05）and the level of 6-keto-PGF1α reduced（P0.05）. The level of NO and the ratio of NO/ET were higher in water extraction than alcohol extraction（P0.05）,whereas the level of 6-ketoPGF1α was（4∶1）lower than alcohol extraction（P0.05）. Conclusion：Each of the medicated groups was effective on the model of migraine with blood stasis and alcohol extractions were more effective than water extractions.

关 键 词：川芎 天麻 偏头痛 血瘀证 

分 类 号：R284[医药卫生—中药学] R747.2[医药卫生—中医学]