salusin-β在麻醉大鼠头端延髓腹外侧区的心血管效应  

Effects of Salusin- β on Blood Pressure and Heart Rate within the Rostral Ventrolateral Medulla in Anesthetized Rats

作　　者：谢富佳[1] 王斌生[1] 李斌[1] 蔡红燕[2] 柴琛[1] 鲁彦[3] 曹农[1] 

机构地区：[1]兰州大学第一医院普外科,730000 [2]兰州大学第二医院重症医学科,730000 [3]中国人民解放军第一医院检验科,兰州730000

出　　处：《医学研究杂志》2016年第2期18-21,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(30700266);甘肃省自然科学基金资助项目(1308RJZA269)

摘　　要：目的在SD大鼠头端延髓腹外侧区(rostral ventrolateral medulla,RVLM)内给予salusin-β,探究其对大鼠心率(heart rate,HR)和血压(blood pressure,BP)的影响。方法雄性SD大鼠32只,随机分为5组,单侧RVLM微量给予谷氨酸(L-glutamic acid,Glu)、人工脑脊液(artificial cerebrospinal fluid,a CSF)或不同剂量的salusin-β(0.04、0.4、4pmol),监测salusin-β对SD大鼠HR和BP的影响。结果大鼠RVLM微量给予a CSF不影响麻醉大鼠的BP 1±1mm Hg,给予Glu血压升高显著10±1mm Hg(P<0.05)。RVLM微量给予salusin-β(0.04、0.4、4pmol)均产生升高血压的作用,且呈剂量依赖性(0.04pmol:6±1mm Hg,P>0.05;0.4pmol:12±1mm Hg,P<0.05;4pmol:29±3mm Hg,P<0.05),且4pmol组与Glu组比较,差异有统计学意义(P<0.05)。但salusin-β对心率无明显影响。结论 salusin-β在SD大鼠RVLM产生剂量依赖性升高BP的作用,但对HR无明显影响。Objective To investigate the effects of salusin -β on blood pressure and heart rate within the rostral ventrolateral medulla in Sprague - Dawley rats. Methods Thirty - two adult male Spargue - Dawley rats were randomly divided into five groups. The dose -dependant effects of salusin - β （0.04, 0.4 and 4pmol, respectively） on blood pressure and heart rate in the RVLM were determined by unilateral microinjection of salusin - β, L - glutamic acid or artificial cerebrospinal fluid （aCSF） into the RVLM. Results Unilateral microinjection of aCSF into the RVLM did not affect blood pressure 1 lmmHg in anesthetized rats, injection Glu significantly elevated blood pressure 10 ＋ lmmHg（P 〈0.05）. Unilateral microinjection of salusin- β into the RVLM produced a dosedependant increase in blood pressure （BP） （0.04pmol： 6 ±3mmHg, P 〉0.05; 0.4pmol： 12 ＋3mmHg, P〈0.05; 4pmol： 29 ±8mmHg, P〈 0. 05） , and with little effect on heart rate （ HR）. Conclusion Salusin -β in RVLM can cause dose - dependant elevation of BP, but no significant effect on HR.

关 键 词：salusin—β 大鼠 头端延髓腹外侧区 血压 心率 

分 类 号：R3[医药卫生—基础医学]