巨细胞病毒宫内感染对子代豚鼠学习记忆的影响研究  

Effect of Learning and Memory Ability in the Descendant Guinea Pig with Cytomegalovirus Intrauterine Infection

作　　者：乔媛[1] 刘静[2] 肖娟[2] 刘涛[3] 李家福[1] 张元珍[1] 陈素华[2] 

机构地区：[1]武汉大学中南医院妇产科,430071 [2]华中科技大学同济医学院附属同济医院妇产科,武汉430030 [3]泰安市中心医院妇产科,271000

出　　处：《医学研究杂志》2016年第2期62-67,共6页Journal of Medical Research

基　　金：武汉大学青年教师基金资助项目(2042015kf0154)

摘　　要：目的观察巨细胞病毒（cytomegalovirus，CMV）宫内感染对子代豚鼠学习记忆的影响。方法PCR法筛选CMVDNA阴性豚鼠，雌雄合笼受孕；孕龄1—20天时，感染组腹腔内接种CMV病毒悬液，对照组接种生理盐水，足月分娩的子代豚鼠分别喂养至3月龄和6月龄，应用Morris水迷宫实验检测学习记忆能力，观察大脑海马组织神经元细胞的形态。结果感染组与对照组相比，子代豚鼠的学习记忆能力明显降低，差异有统计学意义（P〈0．01）；感染组的海马组织神经元细胞结构层次紊乱，出现变性、灶性坏死及凋亡等病理改变。结论CMV宫内感染可以导致子代学习记忆能力的下降。Objective To study the learning and memory of the descendant guinea pig with cytomegalovirus （CMV） intrauterine infection. Methods These female and male guinea pigs without CMV - infected identified by PCR were chosen in this study. The pregnancy was observed after each mating. CMV was injected into abdominal cavity to create CMV intrauterine infection model in gestational age from one to twenty,and saline was injected in control group. These full term offspring were fed for 3months or 6moths respectively. The learning and memory ability was measured by Morris water maze. The changes of modality and apoptosis of hippocampal neurons were observed with light microscope. Results The learning and memory ability of guinea pigs in CMV - infected group was much lower than those in the control group（P 〈 0.01 ）. In CMV - infected group, the layers of hippocampal neurons were confused, and pathologic changes were seen clearly such as degeneration, focal necrosis and apoptotie. Conclusion CMV intrauterine infection could decrease the ability of learning and memory of descendant.

关 键 词：巨细胞病毒 宫内感染 豚鼠 学习记忆 

分 类 号：R714.53[医药卫生—妇产科学]