烯禾啶胁迫对谷子愈伤组织生理特性的影响  

Effects of Sethoxydim stress on physiological characteristics of foxtail millet callus

作　　者：宋惠洁 余凯凯[1] 刘阳[1] 黄蕾[1] 郭平毅[1] 王玉国[1] 温银元[1] 原向阳[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2016年第2期107-110,115,共5页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金："十二五"国家科技支撑计划(2014BAD07B01-09);国家自然基金(31301269);山西省科技攻关项目(20150311016-2);山西省科技攻关项目(20120311004-4);山西农业大学青年拔尖创新人才支持计划(TYIT201406);山西省重点研发计划项目(2015-TN-09)

摘　　要：为了研究烯禾啶胁迫对谷子愈伤组织生长及生理特性的影响,在添加0、0.5、1、2、4 m L·L^(-1)烯禾啶的MS培养基上培养谷子愈伤组织,测定其SOD、POD活性、MDA含量、可溶性蛋白和脯氨酸含量,并对其进行分析。结果表明,随着烯禾啶浓度的增加及胁迫时间的延长,愈伤组织生长逐渐缓慢,烯禾啶浓度为2 m L·L^(-1)时,愈伤组织生长极为缓慢,4 m L·L^(-1)时,愈伤组织基本不生长;SOD、POD活性与可溶性蛋白、脯氨酸含量随着烯禾啶浓度的增加先升高后降低;MDA含量在1 m L·L^(-1)及以下时积累缓慢,高于1 m L·L^(-1)时积累较快。In order to study the effects of Sethoxydim stress on the growth and physiological characteristics of Foxtail Millet, The callus was cultured On MS medium with 0,0.5,1,2,4 mL. L-1 Sethoxydim and the activities of SOD, POD and the contents of soluble proteins, Proline, and MDA were determined and analyzed. The results showed that the callus grew gradually slow with increasing of Sethoxydim concentration and extending of stress time . When the concentration of Sethoxydim was 2 mL.L-1 and the callus was not growing under 4 mL.L-1. The ativities of SOD, POD and the content of soluble protein, proline increased first and then decreased. The content of MDA accumulated slowly when Sethoxydim concentration at the below of 1mL.L -1 , then increased rapidly.

关 键 词：谷子 烯禾啶 愈伤组织 相对生长量 保护酶 渗透调节物质 

分 类 号：S515[农业科学—作物学]