单胺氧化酶抑制剂体外筛选模型的建立  

Establishment of the screening model for monoamine oxidase inhibitors in vitro

作　　者：刘子修[1] 刘梅[1] 马静[1] 邱彦[1] 蔡鹰[1] 陆瑜[1] 

机构地区：[1]解放军第454医院药学部,南京210002

出　　处：《中国临床药学杂志》2016年第1期8-11,共4页Chinese Journal of Clinical Pharmacy

基　　金：南京药学会-常州四药医院药学科研基金项目(编号2012YX008)

摘　　要：目的建立一种可筛选对单胺氧化酶(MAO)有抑制作用药物的体外模型,用于评价药物对单胺氧化酶的影响。方法以5-羟色胺(5-HT)和苄胺分别作为单胺氧化酶A(MAO-A)和单胺氧化酶B(MAO-B)的反应底物,采用紫外分光光度法,分别在280、242 nm测定吸光度,计算药物对酶的抑制率。结果建立的模型筛选了17种药物,其中15种药物对MAO-A有抑制作用,13种药物对MAO-B有抑制作用。结论此模型操作简单,重复性好,可用于大规模体外筛选MAO-A和MAO-B的抑制药物,指导临床合理使用此类制剂。AIM To establish a filterable in vitro model for monoamine oxidase （MAO） inhibitory drugs for eval- uating the effects of drugs on monoamine oxidase. METHODS With 5-hydroxytryptamine （5-HT） and benzylamine as reaction substrates for MAO-A and MAO-B, 280 nm and 242 nm absorbance of UV spectrophotometry was measured to calculate the inhibition rate of the drug. RESULTS A total of 17 drugs were screened, among them, 15 kinds showed MAO-A inhibition,and 13 kinds showed MAO-B inhibition. CONCLUSION This model is simple to operate, and can be used for large-scale screening in vitro MAO-A and MAO-B inhibitory drugs, and to guide the rational use of such preparations.

关 键 词：单胺氧化酶 抑制作用 5-羟色胺 苄胺 

分 类 号：R284[医药卫生—中药学]