北京地区6岁以下儿童急性呼吸道感染病例人博卡病毒流行状况分析  

Human Bocavirus surveillance in children aged < 6 years with acute respiratory tract infection in Beijing

作　　者：吴占国[1] 李爱华[2] 张铁钢[2] 龚成[2] 陈萌[2] 黄芳[2,3] 

机构地区：[1]北京市大兴区疾病预防控制中心,北京102600 [2]北京市疾病预防控制中心,北京100013 [3]首都医科大学,北京100069

出　　处：《疾病监测》2016年第1期24-28,共5页Disease Surveillance

基　　金：国家"十二五"传染病科技重大专项(No.2012ZX1004-206)~~

摘　　要：目的了解北京地区6岁以下儿童急性呼吸道感染病例中人博卡病毒的流行状况,探讨其流行特征。方法2013年1月至2014年12月,从北京市28家哨点医院采集6岁以下急性呼吸道感染病例标本5623份,采用Real-time PCR及巢式RT-PCR方法,检测人博卡病毒核酸和基因分型。结果共检出人博卡病毒38例,阳性检出率为0.68%（38/5623）,不同年龄组的阳性检出率：0~1岁组为0.88%,1~3岁组为1.00%,3~6岁组为0.34%。3岁以下儿童组比3岁以上儿童组人博卡病毒检测阳性率高（χ2=10.058,P=0.001）。不同时间人博卡病毒阳性检出率：2013年3月为0.48%（1/208）,8月为0.90%（2/221）,9月为1.52%（4/263）,10月为2.00%（9/449）,11月为1.52%（8/524）,12月为0.35%（2/567）,其余月未检出;流行季节为8-11月,高峰期为10月;2014年3月为0.19%（1/519）,5月为0.57%（2/348）,8月为5.56%（4/72）,9月为1.14%（1/88）,10月为3.41%（3/88）,其余月未检出;流行季节为8-10月,高峰期为8月。7例病例为人博卡病毒与其他病毒（冠状病毒、鼻病毒、呼吸道合胞病毒、副流感3型病毒）混合感染,混合感染率为18.42%（7/38）。38例病例检出的人博卡病毒基因型别均为HBo V1亚型,与参考株HBo V1 ST1和ST2株核苷酸同源性为98.4%~99.6%,氨基酸同源性为98.2%~100.0%。结论北京地区部分儿童急性呼吸道感染与HBo V相关;北京地区的HBo V以HBo V1亚型为主,流行季节性为8-11月。Objective To investigate the infection status of Human Bocavirus（ HBo V） in children aged 6 years with acute respiratory tract infection in Beijing from 2013 to 2014. Methods A total of 5623 clinical samples were collected from the children aged 6 years with acute respiratory tract infections in 28 sentinel hospitals in Beijing from January 2013 to December 2014. Real time PCR and nest RT-PCR were conducted to detect the nucleic acid of HBo V and subsequent genotyping. Results A total of 38 HBo V infection cases were detected,the overall positive rate was 0. 68%. The positive rate was 0. 88% in age group 0- 1 years,1. 00% in age group 1- 3 years and 0. 34% in age group 3- 6 years. The positive rate was higher in age group 3 years than in age group 3 years（ χ2= 10. 058,P = 0. 001）. In 2013,the positive rate was0. 48% in March（ 1 /208）,0. 90% in August（ 2 /221）,1. 52% in September（ 4 /263）,2. 00% in October（ 9 /449）,1. 52% in November（ 8 /524） and 0. 35% in December（ 2 /567）. In 2014,the positive rate was 0. 19% in March（ 1 /519）,0. 57% in May（ 2 /348）,5. 56% in August（ 4 /72）,1. 14% in September（ 1 /88） and 3. 41% in October（ 3 /88）. The infections mainly occurred during August-October and the incidence peak was in August. The co-infection rate of HBo V and other respiratory viruses（ coronavirus,rhinovirus,respiratory syncytial virus and parainfluenza virus type 3） was 18. 42%（ 7 /38）. The 38 HBo V1 strains shared 98. 4%- 99. 6% homology of nucleotide sequence and 98. 2%- 100. 0% homology of amino acid sequence respectively with prototype strains ST1 and ST2. Results Some acute respiratory tract infections in children in Beijing were associated with HBo V infection,HBo V1 was predominant and the infection usually occurred during August-November in Beijing.

关 键 词：人博卡病毒 急性呼吸道感染 聚合酶链反应 

分 类 号：R725.6[医药卫生—儿科]