高效液相色谱法测定AC4可溶性粉中AC4的含量  

Analytical Method for Determination of AC4 in Soluble Powder by HPLC

作　　者：王汝金[1,2] 薛飞群[1] 王霄旸[1] 张丽芳[1] 郝智慧[2] 

机构地区：[1]中国农业科学院上海兽医研究所兽药安全评价与兽药残留重点开放实验室,上海200241 [2]青岛农业大学化学与药学院,山东青岛266109

出　　处：《中国家禽》2016年第3期19-23,共5页China Poultry

基　　金：公益性行业(农业)科研专项(201303038);国家高技术研究发展计划(863计划)(2011AA10A214)

摘　　要：研究旨在建立一种测定可溶性粉剂中AC4含量的高效液相色谱方法.采用C18色谱柱,流动相为甲醇和0.2％磷酸水溶液（v/v,65：35）,流速为1 mL/min,柱温为30℃,紫外检测波长为251 nm.该方法专属性好,具有良好的线性关系,线性范围为0.07～0.13 mg/mL,R2=0.9987,AC4的平均添加回收率为98.45％,相对标准偏差为0.5044％,24h内稳定性良好.该方法操作简单,精密度及重现性生好,可用于可溶性粉剂中AC4的含量测定.This study aimed to establish a high performance liquid chromatography （HPLC）method for the determination of AC4 content in soluble powder. Determination was carried out by means of a C18 column with the column temperature at 30℃;The mobile phase consisted of methanol and 0.2% phosphoric acid solution （v/v,65：35）with the flow rate of 1 mL/min. The UV detector was operated at 251 nm. The method displayed a good performance at specificity. Good linear relationships with high correlation coefficients （R2=0.9987）were obtained when the AC4 concentration ranged from 0.07 mg/mL to 0.13 mg/mL. The average recovery of AC4 and the relative standard deviation were 98.45% and 0.5044% respectively,and AC4 kept stable within 24 h. With the characteristics of convenience,accuracy and repeatability, the method was allowed to be used for the determination of AC4 content in soluble powder.

关 键 词：高效液相色谱法 可溶性粉 AC4 

分 类 号：S859.79[农业科学—临床兽医学] O657.7[农业科学—兽医学]