乙氧氟草醚对大鳞副泥鳅的毒性测定  

Toxic Determination of Oxyfluorfen on Paramisgurnus dabryanus

作　　者：夏晓华[1] 董慧[1] 李会娜 夏晓培 常重杰[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南省汝州市质量技术监督检验测试中心,河南汝州467500

出　　处：《贵州农业科学》2016年第1期113-116,共4页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"两种泥鳅DMRT1基因不同选择性剪接体的结构;功能及调控机制研究"(31200923);河南省教育厅科学技术研究重点项目"大鳞副泥鳅DMRT1基因不同选择性剪接体的表达模式分析"(12B180011);河南师范大学引进人才科研项目"泥鳅DMRT1基因不同剪接体的克隆和时空表达模式分析"(01046500109)

摘　　要：为探明除草剂乙氧氟草醚(Oxyfluorfen)对水生生物的毒性,以大鳞副泥鳅(Paramisgurnus dabryanus)为受试对象,进行乙氧氟草醚对大鳞副泥鳅的急性毒性、生理毒性和DNA损伤试验。结果表明:随着染毒浓度增加和时间的延长,大鳞副泥鳅的死亡率升高,安全浓度为8.40mg/L,根据中国《化学农药环境安全评价试验准则》,乙氧氟草醚对大鳞副泥鳅为低毒。乙氧氟草醚低浓度(8.00 mg/L,10.50mg/L,13.00mg/L)作用下,大鳞副泥鳅肝脏谷氨酸草酰乙酸转氨酶(GOT)、谷氨酸丙酮酸转氨酶(GPT)活性明显升高;高浓度(15.50mg/L)作用下酶活性在染毒2d、4d时呈上升趋势,但在6d时急速下降。15.50mg/L组与空白对照组相比,大鳞副泥鳅肝细胞的彗尾DNA百分含量、彗星尾长和Olive尾矩明显增加,肝细胞受到明显损伤。In order to assess the toxicity of herbicide oxyfluorfen to aquatic organisms,regarding P.dabryanus as the experimental object,acute toxicity,physiological toxicity and DNA damage experiment were employed to study oxyfluorfen＇s effect on P.dabryanus.Results：P.dabryanus mortality increased with the concentration increasing and the extension of time.The activities of GPT and GOT in P.dabryanus liver cells were induced by the lower concentration（8.00mg/L,10.50mg/L,13.00mg/L）treatments group.The action of enzyme activity on 2d,4drises,but it fell sharply in the 6dunder the high concentration（15.50mg/L）of the oxyfluorfen.The liver cells＇ DNA damage were checked by single cell gel electrophoresis technique（SCGE）.Compared with the control group,treatment group＇s（15.50mg/L）three indexes,tail DNA content,the comet tail length and olive tail moment increased significantly（P0.05）,liver cell was obviously damaged.

关 键 词：乙氧氟草醚 大鳞副泥鳅 谷丙转氨酶 谷草转氨酶 单细胞凝胶电泳 

分 类 号：S948[农业科学—水产养殖]