棘孢木霉对黄花蒿叶的光合特性和产量影响  

Effects of Trichoderma asperellum on Leaf Photosynthetic Characteristics and Yield of Artemisia annua

作　　者：杨兴堂[1] 吕曼曼[1] 刘志华[2] 朱国栋[1] 王慧[1] 马德志[3] 张荣沭[1] 

机构地区：[1]东北林业大学园林学院,黑龙江哈尔滨150040 [2]东北林业大学林学院,黑龙江哈尔滨150040 [3]齐齐哈尔医学院,黑龙江齐齐哈尔161006

出　　处：《贵州农业科学》2016年第1期132-136,共5页Guizhou Agricultural Sciences

基　　金：国家自然科学基金资助项目"木霉诱导下杨树ARF转录因子对其生长及抗病的分子调控机制"(31370642);国家自然科学基金资助项目"棘孢木霉的刺激植物响应蛋白Epl1诱导杨树系统抗病性机制"(31170601);黑龙江省自然科学基金资助项目"木霉菌促进山新杨生长的Auxin信号转导机制"(C201216)

摘　　要：为了对黄花蒿的增产和促进木霉免疫诱导剂类生物肥料在植物生长中的应用提供理论依据,在大田条件下进行根施1×105cfu/mL(T1)、1×106 cfu/mL(T2)和1×107cfu/mL(T3)3个水平的棘孢木霉ACCC30536分生孢子对黄花蒿叶的光合特性和产量的影响试验。结果表明:T3水平(200mL/株)的木霉菌对黄花蒿的诱导效果最佳;根施木霉孢子量与青蒿叶片净光合速率(Pn)呈正相关,光合"午休"现象有一定程度的减缓;T3处理黄花蒿的光合-光响应曲线参数最大净光合速率、表观量子效率、暗呼吸速率及光饱和点、光补偿点均高于CK和其他处理。并且,木霉菌诱导黄花蒿60d后T3组黄花蒿叶的产量较CK提高最大。说明,棘孢木霉ACCC30536能够改善黄花蒿的光合能力,促进干物质的积累,从而提高其叶的产量。The photosynthetic characteristics and leaf yield of Artemisia annua respectively applying with 1 105cfu/mL（T1）,1×106 cfu/mL（T2）and 1×107cfu/mL（T3）T.asperellum ACCC30536 conidium by root application in field were studied to provide the theoretical basis for increasing Artemisia annua yield and promoting application of Trichoderma as an immune-inducing biofertilizer in plant growth.Results：The inductive effect of T3 treatment on Artemisia annuais the best among three treatments.The application amount of Trichoderma asperellum spores is positively related to Pn of Artemisia annua leaves.Applying Trichoderma asperellum can retard the photosynthetic ＂noon break＂phenomenon to some extent.The maximum net photosynthetic rate,apparent quantum yield,dark respiratory,light saturation point and light compensation point of Artemisia annua treated with 1 107 cfu/mL（T3）T.asperellum ACCC30536 conidium all are higher than CK,T1 and T2treatment.The Artemisia annualeaf yield of T3 treatment increases significantly compared with CK after 60 d.In conclusion,applying T.asperellum ACCC30536 can improve the photosynthetic capacity of Artemisia annua and promote dry matter accumulation,which results in yield increase of Artemisia annualeaf.

关 键 词：棘孢木霉 黄花蒿 光合特性 光响应 叶产量 

分 类 号：S567.219[农业科学—中草药栽培]