Potential natural exposure of endangered red-crowned crane(Grus japonensis) to mycotoxins aflatoxin B_1, deoxynivalenol, zearalenone, T-2 toxin, and ochratoxin A  

盐城生物圈保护区越冬丹顶鹤受到真菌毒素威胁的研究（英文）

作　　者：Da-wei LIU Hong-yi LIU Hai-bin ZHANG Ming-chang CAO Yong SUN Wen-da WU Chang-hu LU 

机构地区：[1]Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China [2]Yancheng Biosphere Reserve, Yancheng 224057, China [3]College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China [4]Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing 210042, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2016年第2期158-168,共11页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the National Natural Science Foundation of China(No.31402268);the Natural Science Foundation of Jiangsu Province of China(Nos.BK20140691 and BK2011083);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China

摘　　要：A survey was conducted to determine whether mycotoxins were present in the foods consumed by red-crowned cranes（Grus japonensis） in the Yancheng Biosphere Reserve, China. Collected in the reserve＇s core, buffer, and experimental zones during overwintering periods of 2013 to 2015, a total of 113 food samples were analyzed for aflatoxin B1, deoxynivalenol, zearalenone, T-2 toxin, and ochratoxin A using high performance liquid chromatography（HPLC）. The contamination incidences vary among different zones and the mycotoxins levels of different food samples also presented disparity. Average mycotoxin concentration from rice grain was greater than that from other food types. Among mycotoxin-positive samples, 59.3% were simultaneously contaminated with more than one toxin. This study demonstrated for the first time that red-crowned cranes were exposed to mycotoxins in the Yancheng Biosphere Reserve and suggested that artificial wetlands could not be considered good habitats for the birds in this reserve, especially rice fields.目的:调查在盐城生物圈保护区越冬的丹顶鹤食物是否受到真菌毒素污染以及评估丹顶鹤受到真菌毒素威胁的风险。创新点:首次证明在盐城生物圈保护区越冬的丹顶鹤受到真菌毒素的威胁。方法:2013年11月至2015年3月,两个越冬期内在盐城生物圈保护区采集丹顶鹤不同觅食生境内113份食物样品。使用高效液相色谱法对这些食物中毒素(黄曲霉毒素B1(AFB1)、脱氧雪腐镰刀菌烯醇(DON)、玉米赤霉烯酮(ZEN)、T-2毒素(T-2)和赭曲霉毒素A(OTA))含量进行检测。结论:盐城生物圈保护区内丹顶鹤食物受到真菌毒素的污染,人工湿地尤其是稻田不适宜作为鸟类的觅食地。

关 键 词：Food MYCOTOXIN Red-crowned crane Yancheng Biosphere Reserve 

分 类 号：S858.93[农业科学—临床兽医学]