可溶性CD40配体对K562细胞增殖及生存素mRNA和IL-8表达的影响  

Effect of soluble CD40 ligand on proliferation of K562 cells and expression level of survivin mRNA

作　　者：封忠昕[1] 吴燕梅 陈琦[3] 肖建辉 冯进[1] 

机构地区：[1]遵义医学院附属医院检验科,贵州遵义563003 [2]菏泽市立医院老年科,山东274031 [3]遵义医学院附属医院血液内科,贵州遵义563003 [4]贵州省细胞工程重点实验室,遵义563003

出　　处：《现代医药卫生》2016年第4期481-483,486,共4页Journal of Modern Medicine & Health

基　　金：贵州省社会发展攻关项目(黔科合SY字[2014]3025号)

摘　　要：目的探究可溶性CD40配体(s CD40L)对人白血病K562细胞增殖、生存素(survivin)m RNA表达和白介素8(IL-8)表达水平的影响。方法采用噻唑蓝(MTT)法检测s CD40L对K562细胞增殖的影响,通过RT-PCR检测survivin m RNA表达水平,采用酶联免疫吸附试验(ELISA)测定IL-8表达情况。结果不同浓度的s CD40L(0.5、1.0、1.5、2.0、2.5μg/m L)对K562细胞增殖均有抑制作用,且可降低survivin m RNA及IL-8的表达水平;s CD40L细胞组细胞增殖抑制率高于K562细胞组,survivin m RNA及IL-8表达水平均低于K562细胞组,差异均有统计学意义(P<0.05),且呈剂量-时间依赖性。结论 s CD40L在体外能够明显抑制K562细胞增殖,具有剂量-时间依赖性,s CD40L抑制K562细胞增殖可能与survivin m RNA及IL-8表达水平下调相关。Objective To investigate the effect of soluble CD40 ligand（s CD40L） on the proliferation of human leukemia K562 cells and expression levels of surviving m RNA and IL-8. Methods The MTT assay was used to examine the effect of different concentrations of s CD40 L on the cellular proliferation,RT-PCR was used to determine the expression level of survivin m RNA;ELISA was used to detect the IL-8 expression. Results Different concentrations of s CD40L（0.5,1.0,1.5,2.0,2.5 μg/m L） all had the inhibiting effect on K562 cell proliferation,moreover could decrease the expression levels of survivin and IL-8;the cellular proliferation inhibiting rate of the s CD40 L cell group was higher than that of the K562 cell group,while the expression levels of surviving m RNA and IL-8 were lower than those in the K562 cell group,the difference was statistically significant（P〈0.05）,moreover which showed the dose and time dependent manner. Conclusion s CD40 L can significantly inhibit the proliferation of K562 cells in vitro with the dose and time dependent manner,s CD40 L inhibiting the K562 cell proliferation is correlated with the down-regulation of surviving m RNA and IL-8 expression.

关 键 词：K562细胞 细胞增殖 SURVIVIN基因 白细胞介素8 可溶性CD40配体 

分 类 号：R971.1[医药卫生—药品]