高速逆流色谱法分离纯化龙胆碱性乙醇提取物中的龙胆碱  

HSCCC separation and purification gentian alkaline ethanol extracts of gentian

作　　者：石垚[1] 王琳琳[1] 余河水[1] 赖青海 丁辉[1] 张丽娟[1] 宋新波[1] 

机构地区：[1]天津中医药大学,天津300193

出　　处：《天津中医药大学学报》2016年第1期44-47,共4页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：天津市科技计划资助项目(12TXGCCX03800)

摘　　要：[目的]建立从龙胆碱性乙醇提取物中分离龙胆碱的高速逆流色谱(HSCCC)方法。[方法]采用高速逆流色谱仪分离纯化龙胆碱性乙醇提取物中的龙胆碱,通过优化溶剂系统,以正己烷∶乙酸乙酯∶甲醇∶水(5∶5∶4∶6)为溶剂体系,上相为固定相,下相为流动相,仪器转速为850 r/min,流速为1.5 m L/min,温度为25℃,检测波长为280 nm。[结果]龙胆碱性乙醇提取物经过D151弱酸性阳离子交换树脂富集(20%乙醇洗脱部分),再采用HSCCC技术进行分离纯化,制备得到的98.8%纯度的龙胆碱。[结论]该方法稳定、回收率高,可高效地从龙胆碱性乙醇提取物中分离龙胆碱。[Objective] To establish the HSCCC method for separating the gentianine from the alkaline ethanol extracts of gentiana. [Methods] Separation and purification of gentianine by TBE300B HSCCC technology, through the optimization of the solvent system, with n-hexane ： ethyl acetate ： methanol ： water （5：5：4：6） as the solvent system, the upper as stationary phase and the next phase as mobile phase, the rotational speed of the instrument was 850 r/rain, the flow rate was 1.5 mL/min, the temperature was 25 ~C, the detection wavelength was 280 nm. [Results] The gentianine was obtained from the gentian alkaline ethanol extracts by D151 cation exchange resin combining with HSCCC, the content was above 98.8%. [Conclusion] The method is stable and has high results, it could he used to separate gentianine efficiently from alkaline ethanol extract of gentiana.

关 键 词：龙胆 龙胆碱 高速逆流色谱 

分 类 号：R284.2[医药卫生—中药学]