骨髓间充质干细胞逆转支气管哮喘小鼠气道重构及其机制的初步研究  

Preliminary study on reversing airway remodeling in asthmatic mice by bone marrow mesenchymal stem cells and its mechanism

作　　者：刘仕欣[1] 颛孙永勋[2] 谭磊[2] 

机构地区：[1]广东省增城市人民医院(中山大学孙逸仙纪念医院增城院区)呼吸内科,广州511300 [2]中山大学孙逸仙纪念医院呼吸内科,广州510120

出　　处：《国际呼吸杂志》2016年第2期81-85,共5页International Journal of Respiration

基　　金：广东省医学科研基金（B2014128）

摘　　要：目的：探讨骨髓间充质干细胞逆转支气管哮喘小鼠气道炎症及对气道重构的影响。方法：采用40只雌性的BALB/c小鼠，随机分组为骨髓间充质干细胞对照组、骨髓间充质干细胞治疗组、哮喘模型组、正常对照组。分离雄性4周龄的小鼠中分离出骨髓间充质干细胞。应用卵白蛋白制备慢性哮喘的小鼠模型，采用流式细胞计数法检测小鼠外周血中CD4＋、CD25＋调节性T细胞的情况，并观察骨髓间充质干细胞抑制之后气道的重构及气道炎症的情况。结果：病理检查可见哮喘模型支气管上皮黏膜有杯状细胞增生，上皮黏膜脱落，部分管腔有大量的炎性细胞浸润，气道平滑肌细胞肥大增生；正常对照组与骨髓间充质干细胞对照组无气道重构及炎症表现，而骨髓间充质干细胞治疗组有气道炎症并且气道重构现象明显降低。哮喘模型组的CD4＋、CD25＋调节性T细胞占淋巴细胞比例比骨髓间充质干细胞对照组及正常对照组明显降低（P＜0.05）；骨髓间充质干细胞治疗组CD4＋、CD25＋调节性T细胞占淋巴细胞比例比哮喘组明显上升（P＜0.05）；而骨髓间充质干细胞治疗组、骨髓间充质干细胞对照组、正常对照组之间无统计学差异。结论：骨髓间充质干细胞移植可逆转支气管哮喘小鼠的气道炎症和气道重构的程度，通过上调CD4＋、CD25＋调节性T细胞的比例发挥作用的。Objective： To investigate the bone marrow mesenchymal stem cells to reverse asthmatic airway inflammation and airway remodeling effect. Methods： 40 female BALB / c mice were randomly divided into bone marrow mesenchymal stem cells in the control group, bone marrow mesenchymal stem cells in the treatment group, asthma model group and normal control group. 4-week-old male mice were isolated isolated bone marrow mesenchymal stem cells. Preparation of ovalbumin mouse model of chronic asthma, the use of flow cytometry to detect splenic tissue ＋, CD25 ＋ regulatory T cells in the case of CD4, and observed after suppression of bone marrow stem cells to rebuild and airway inflammation in the airways of the case. Results： Pathological examination showed asthmatic bronchial epithelial mucosa goblet cell hyperplasia, mucosal epithelial shedding, a large part of the bureaucratic inflammatory cell infiltration, airway airway smooth muscle cells hypertrophy; normal control group and the mesenchymal stem cell control None Airway remodeling and inflammation, and bone marrow mesenchymal stem cells in the treatment group and airway inflammation and airway remodeling significantly reduced. Asthma model group of CD4 ＋, CD25 ＋ regulatory T cells in lymphocytes than bone marrow mesenchymal stem cells in the control group and the normal control group was significantly lower （P 〈0.05）; bone marrow mesenchymal stem cells treated CD4 ＋, CD25 ＋ regulatory T cells accounted for lymphocyte ratio increased significantly than that of asthma group （P〈0.05）; and bone marrow mesenchymal stem cells in the treatment group, bone marrow mesenchymal stem cells in the control group, no significant difference between the control group. Conclusion： Bone marrow mesenchymal stem cells can reverse asthmatic mice degree of airway inflammation and airway remodeling through upregulation CD4 ＋, CD25 ＋ regulatory T cells play a role in proportion.

关 键 词：骨髓间充质干细胞 支气管哮喘 气道重塑 气道炎症 

分 类 号：R562.25[医药卫生—呼吸系统]