血清和肺组织匀浆HIF-1α水平在COPD稳定期合并周围型肺癌中的变化和意义研究  

Change and significance of HIF-1α level in stable COPD combined peripheral lung cancer

作　　者：鞠鑫鹏[1] 孙丽凤 王东升[3] 

机构地区：[1]新汶矿业集团莱芜中心医院呼吸科,莱芜271100 [2]烟台市中医医院肺病科264000 [3]吉林大学朝阳校区医院内科,长春130033

出　　处：《国际呼吸杂志》2016年第2期108-110,共3页International Journal of Respiration

摘　　要：目的：探讨血清和肺组织低氧诱导因子- 1（Hypoxia inducible factor – 1，HIF-1α）水平在慢性阻塞性肺疾病（chronic obstructive pulmonary，COPD）稳定期合并周围型肺癌患者中的变化和意义。方法：选取周围型肺癌择期手术患者76例，根据术前是否具有COPD合并症将患者分为两组，其中COPD稳定期作为A组（38例），肺功能正常者作为B组（38例），术中采集肺癌及其癌旁正常肺组织标本，免疫组化法检测肺组织HIF-1α表达，ELISA方法检测血清和肺组织匀浆HIF-1α水平，分析HIF-1α与肺功能指标（肺活量VC、一秒用力呼气量FEV1、残气量/肺总量RV/TCL）的关系。结果：A组血清HIF-1α、肺组织匀浆HIF-1α和肺组织HIF-1α阳性表达率均明显高于B组，两组比较差异具有统计学意义（P〈0.05），肺癌组织HIF-1α阳性表达率明显高于同组正常肺组织（P〈0.05），经Pearson相关性分析发现，血清和肺组织匀浆HIF-1α与VC、FEV1呈负相关，与RV/ TCL呈正相关。结论：血清和肺组织HIF-1α在COPD稳定期合并周围型肺癌中明显增高，可能与肺功能的异常改变相关。Objective To investigate changes and significance of the serum and lung tissue hypoxia inducible factor-is （HIF-1α） levels in patients with stable chronic obstructive pulmonary disease （COPD） complicated with peripheral lung cancer. Methods Seventy-six patients of peripheral lung cancer undergoing operation were selected, lung cancer and its adjacent normal lung tissue specimens were collected during operation, according to whether with COPD, patients were divided into two groups, stable COPD patients were as A group （38 cases）, normal pulmonary function were as group B （38 cases）, lung cancer tissue samples were collected during the operation, HIF-1α express in lung tissue was detected by immunohistochemistry, serum and lung homogenate HIF-1α levels were detected by ELISA, the relationship between HIF-1α and lung [unction index （VC, FEVI, RV/TCL） were analyzed. Results The serum and lung tissue homogenate HIF-1α in A groups were significantly higher than B group （ t =4.36, 5.01, P 〈0.05）, lung cancer tissue positive express rate of HIF-1α in A groups was significantly higher than B group （ χ^2 =6.12, P 〈0.05）, the Pearson correlation analysis showed, the serum and lung tissue homogenate HIF-1α were negatively correlated with and VC, FEV1 （r =-0.681,-0.712,-0.546, -0. 623, P 〈0.05）, the serum and lung tissue homogenate HIF-1α were and positively related to RV/ TCL （ r =0.651,0.611, P 〈0.05）. Conclusions The serum and lung tissue of HIF-1α are increased significantly in SCOPD combined peripheral lung cancer, and may be related to lung function abnormalchanges.

关 键 词：低氧诱导因子1Α 慢性阻塞性肺疾病 周围型肺癌 

分 类 号：R563.9[医药卫生—呼吸系统] R734.2[医药卫生—内科学]