多重耐药肺炎克雷伯菌噬菌体KP002的生物学特性及基因组学研究  

Biological characterization and genome sequence of KP002,a novel bacteriophage isolated from multiple-drug resistant Klebsiella pneumonia

作　　者：高晶[1,2] 徐彬[3] 郭晓奎[1] 秦金红[1] 

机构地区：[1]上海交通大学医学院,上海200025 [2]复旦大学附属妇产科医院,上海200011 [3]上海交通大学医学院附属新华医院崇明分院,上海202150

出　　处：《微生物与感染》2016年第1期18-23,共6页Journal of Microbes and Infections

基　　金：国家科技支撑计划(2012EP001004)

摘　　要：以上海某些医院临床分离到的多重耐药肺炎克雷伯菌为宿主菌,从不同环境的污水中分离获得1株肺炎克雷伯菌噬菌体KP002。电子显微镜显示其为有尾噬菌体,头部直径约70nm,尾长约80nm,尾宽约20nm。对其生物学特性进行研究,结果显示此株噬菌体在pH 3-9及4-50℃的环境中具有较高活性;6min吸附率达95%以上;潜伏期为10min,爆发期为50min;裂解量为172pfu/cell。结果表明,该噬菌体对pH值和温度适应范围较宽。对其全基因组进行测序分析,结果显示其基因组为环状双链DNA,全长47 173bp,GC含量为48%。本研究筛选获得1株对pH值和温度适应范围较宽的耐药肺炎克雷伯菌烈性噬菌体KP002,为建立耐药肺炎克雷伯菌的噬菌体库以用于治疗临床多重耐药菌感染提供了新的思路。Multiple drug-resistant Klebsiella pneumonia strains collected from several hospitals in Shanghai were used as the host bacteria to isolate phages in the wastewater.A novel phage KP002 was isolated and analyzed in details.The morphology and size of the bacteriophage was observed by electron microscope with negative staining.The results indicated that it was a tailed phage with head diameter about 70 nm,tail length about 80 nm and tail width about 20 nm.This phage had high activity at pH from 3to 9and temperature from 4to 50℃.The adsorption rate was above 95% within 6min.Its incubation period was 10 min and burst period was 50 min.The burst size reached 172pfu/cell.The whole genome sequence showed that it contained a circular double-stranded DNA with a full-length of 47 173 bp and GC content of 48%.KP002 is a new phage against drug-resistant bacteria and could be served as a potential resource for clinical treatment of multiple-drug resistant bacterial infection.

关 键 词：多重耐药肺炎克雷伯菌 噬菌体 基因组学 

分 类 号：R440[医药卫生—诊断学]