高效利用内醚糖微生物的筛选鉴定及预处理对菌株利用热解液的影响  

Isolation and Identification of Efficient Levoglucosan-Assimilating Microorganisms and Effects of Pretreatment on their Utilization of Pyrolysis Liquids

作　　者：孙欢[1] 李东晓[1] 陈育如[1] 赵乙萱[1] 刘军利[2] 卫民[2] 

机构地区：[1]南京师范大学生命科学学院,江苏省微生物与基因组学重点实验室,江苏省微生物资源产业化工程技术研究中心,江苏省生物多样性与生物技术重点实验室,江苏南京210023 [2]中国林业科学院南京林产化学工业研究所,江苏南京210042

出　　处：《高校化学工程学报》2016年第1期127-132,共6页Journal of Chemical Engineering of Chinese Universities

基　　金：江苏省高校自然科学研究重大项目(15KJA210002);江苏省“六大人才高峰”人才培养项目(NY-011-2015)

摘　　要：以纤维素热解产物内醚糖为碳源筛选到4株高效利用内醚糖的微生物,经ITS测序和菌落形态特征鉴定,4株菌分别为嗜松青霉(Penicillium pinophilum)、桔青霉(Penicillium citrinum)、冠突散囊菌(Eurotium cristatum)和红假单胞菌(Rhodopseudomonas sp)。对各菌株利用内醚糖的研究结果表明,嗜松青霉和桔青霉5 d内对2%内醚糖的利用率为100%,冠突散囊菌7 d内对2%内醚糖的利用率为100%,红假单胞菌在10 d对2%内醚糖的利用率为52%。将4株菌作用于热解液发现,红假单胞菌能在未经预处理的热解液中生长,14 d后对热解液中内醚糖的利用率为69%,另外3株菌能在经5%活性炭+Ca(OH)2预处理后的热解液中生长。对预处理后热解液成分的分析表明,预处理对热解液成分有显著影响,其中六甲基环三氧硅烷、1,2-环戊二酮、5-甲基糠醛、3-甲基-1,2-环戊二酮和羟甲基呋喃酮的去除率达100%,内醚糖的损失为27.1%。Four efficient levoglucosan-assimiliating microorganisms were screened using levoglucosan from cellulosis pyrolysis as carbon source, and they were identified as Penicillium pinophilum, Penicillium citrinum, Eurotium cristatum and Rhodopseudomonas sp based on morphology and ITS sequence analysis. The results show that the 2%-levoglucosan utilization efficiency of P. pinophilum, P. citrinum（4 d） and E. cristatum（7 d） are 100%, and that of Rhodopseudomonas sp is 52%（10 d）. Rhodopseudomonas sp can be cultured in untreated pyrolysis liquids with utilization efficiency of 69%（14 d）, while Penicillium pinophilum, Penicillium citrinum and Eurotium cristatum can be cultured in pretreated pyrolysis liquids（treated by 5% activated carbon＋Ca（OH）2）. The pretreated pyrolysis liquids were analyzed and the results show that pretreatment has obvious influence on the pyrolysis liquid. Hexamethylcyclotrisiloxane,1,2-Cyclopentanedione,5-methylfurfural, 3-methyl-1,2-Cyclopentanedione and hydroxymethylfuraneol are fully removed, and the loss rate of levoglucosan is 27.1%.

关 键 词：内醚糖 热解液 嗜松青霉 桔青霉 红假单胞菌 

分 类 号：Q935[生物学—微生物学]