HPLC法同时测定三味龙胆花片中龙胆苦苷及獐牙菜苦苷  

Simultaneous determination of gentiopicroside and swertiamarin in Sanwei Longdanhua Tablets by HPLC

作　　者：余继英[1] 吴亮[2] 徐傅能 张静波 陈瑾[1] 何林[1] 

机构地区：[1]四川省医学科学院,四川省人民医院药学部,四川成都610072 [2]西南交通大学,四川成都610000 [3]四川宇妥藏药药业有限公司,四川成都610000

出　　处：《中成药》2016年第2期318-321,共4页Chinese Traditional Patent Medicine

基　　金：四川省科技支撑计划(2012SZ0182)

摘　　要：目的建立HPLC法同时测定三味龙胆花片（白花龙胆、甘草、蜂蜜）中龙胆苦苷及獐牙菜苦苷的含有量。方法药物甲醇提取液的分析采用Diamonsil C18色谱柱（250 mm×4.6 mm,5μm）;柱温30℃;流动相为甲醇-水（25∶75）;体积流量1.0 m L/min;进样量10μL;检测波长240 nm。结果龙胆苦苷和獐牙菜苦苷分别在20.1~201.0 ng（r=0.999 9）和19.76~197.6 ng（r=0.999 9）范围内有良好的线性关系,平均加样回收率分别为99.93%和100.8%,RSD值分别为1.65%和2.06%。结论该方法简便、准确、可靠,可用于三味龙胆花片的质量控制。AIM To establish an HPLC method for the simultaneous determination of gentiopicroside and swertiamarin in Sanwei Longdanhua Tablets（ Gentian algida,Glycyrrhiza uralensis and honey） by HPLC. METHODS The analysis of methanolic extract of this drug was performed on Diamonsil C18column（ 250 mm × 4. 6 mm,5 μm）,with column temperature maintained at 30 ℃,methol-H2O（ 25 ∶ 75） as mobile phase at a flow rate of1 m L / min and detection wavelength set at 240 nm. RESULTS The linear ranges were 20. 1-201. 0 ng（ r = 0. 999 9） for gentiopicroside and 19. 76-197. 6 ng（ r = 0. 999 9） for swertiamarin,whose average recoveries were 99. 93% and 100. 8% with the RSDs of 1. 65% and 2. 06%,respectively. CONCLUSION This simple and accurate method is reliable for the quality control of Sanwei Longdanhua Tablets.

关 键 词：三味龙胆花片 龙胆苦苷 獐牙菜苦苷 HPLC 

分 类 号：R927.2[医药卫生—药学]