HPLC法同时测定淡竹叶中绿原酸和牡荆素的含量  

Contents Determination of Chlorogenic Acid and Vitexin in Lophatherum gracile by HPLC

作　　者：时海燕[1] 徐男 王玉团[3] 苏乐群[1] 李宏建[1] 

机构地区：[1]山东省千佛山医院,济南250014 [2]山东中医药研究院/国家中医药管理局中药分析重点学科,济南250014 [3]山东省食品药品检验所,济南250101

出　　处：《中国药房》2016年第6期833-835,共3页China Pharmacy

摘　　要：目的：建立同时测定淡竹叶中绿原酸和牡荆素含量的方法。方法：采用高效液相色谱法。色谱柱为Waters Atlantis C18,流动相为乙腈-水（梯度洗脱）,流速为1.0 ml/min,检测波长为280 nm,柱温为35℃,进样量为10μl。结果：绿原酸和牡荆素检测进样量线性范围分别为0.041 0~1.228 8（r=0.999 8）、0.264 0~7.920 0μg（r=0.999 9）;精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为97.6%~102.3%（RSD=1.85%,n=9）、97.6%~101.3%（RSD=1.19%,n=9）。结论：该方法操作简便、稳定、重复性好,可用于淡竹叶中绿原酸和牡荆素含量的同时测定。OBJECTIVE：To establish a method for the simultaneous determination of chlorogenic acid and vitexin in Lophatherum gracile. METHODS：HPLC was performed on the column was Waters Atlantis C18 with mobile phases of acetonitrile- water（gradient elution）at a flow rate of 1.0 ml/min,detection wavelength was 280 nm,column temperature was 35 ℃,and the injection volume was 10 μl. RESULTS：The linear range was 0.041 0-1.228 8 μg for chlorogenic acid（r=0.999 8）and 0.264 0-7.920 0μg for vitexin（r=0.999 9）;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were97.6%-102.3%（RSD=1.85%,n=9）and 97.1%-101.3%（RSD=1.19%,n=9）,respectively. CONCLUSIONS：The method is simple,stable and reproducible,and can be used for the simultaneous determination of chlorogenic acid and vitexin in L. gracile.

关 键 词：淡竹叶 绿原酸 牡荆素 含量测定 

分 类 号：R927.2[医药卫生—药学]