柑橘品种对不同基因型柑橘衰退病毒的抑制及变异的影响  

Suppression and Sequence Variation of Citrus tristeza virus Genotypes by Citrus Cultivars

作　　者：唐萌[1] 金鑫[1] 周常勇[1] 李中安[1] 陶珍珍[1] 周彦[1] 

机构地区：[1]西南大学柑橘研究所,中国农业科学院柑橘研究所,国家柑橘工程技术研究中心,重庆400712

出　　处：《园艺学报》2016年第1期55-60,共6页Acta Horticulturae Sinica

基　　金：重庆市两江学者项目;重庆市基础与前沿研究计划重点项目(CSTC2015JCYJBX0043);中央高校基本科研业务费项目(XDJK2015A009);重庆市应用开发计划项目(CSTC2014YYKFA8005)

摘　　要：为研究不同柑橘品种对柑橘衰退病毒(Citrus tristeza virus,CTV)不同基因型的抑制效果,将T36、T30、VT和T3等基因型CTV的毒源分别接种于‘赛蒙斯’甜橙、‘邓肯’葡萄柚、‘墨西哥莱檬’和‘强徳勒柚’,运用RT-qPCR技术检测被接种植株中CTV复制水平的差异。结果表明,‘墨西哥莱檬’最适于CTV各基因型的复制。‘邓肯’葡萄柚中VT、T3和T36基因型的复制水平最低。4种柑橘品种对T36基因型CTV都有明显的抑制作用。通过对CTV的CP基因进行分析发现,VT基因型CTV在‘赛蒙斯’甜橙中发生了较大变异。For the preliminary study on the suppression of Citrus tristeza virus（CTV）genotypes by different citrus cultivars. Four genotypes of CTV were graft-inoculated to ‘Symons＇ Sweet orange,‘Mexican Lime＇,‘Duncan＇ grape fruit and ‘Chandler Pummelo＇. The results of RT-qPCR showed that ‘Mexican Lime＇was most suitable for the survival of these four CTV genotypes. The cope levels of VT,T3 and T36 genotypes in‘Duncan＇grape fruit were much more lower than in other citrus cultivars. Furthermore,T36 genotype was reduced significantly by all of the citrus cultivars. Sequence analysis indicated that CP gene of VT genotype had frequent mutation in sweet orange.

关 键 词：柑橘 品种 柑橘衰退病毒 基因型 RT-QPCR 序列分析 

分 类 号：S666[农业科学—果树学]