冬凌草转录组SSR位点分析及多态性初步评价  

Analysis of SSR Loci Information in Transcriptome of Rabdosia rubescens(Henmsl.)Hara and Its Polymorphism

作　　者：苏秀红[1] 李庆磊[1] 陈随清[1] 董诚明[1] 杨晶凡[1] 尹雷[1] 

机构地区：[1]河南中医药大学药学院,河南郑州450046

出　　处：《北方园艺》2016年第4期93-96,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(81173486);河南中医学院创新人才资助项目(2011XCXRC02);河南中医学院博士科研基金资助项目(BSJJ2009-10);河南省高等学校青年骨干教师计划资助项目(2011GGJS-089);河南省教育厅科学技术研究重点资助项目(13A360613)

摘　　要：以冬凌草转录组为研究对象,使用Microsatellite(MISA)软件进行SSR搜索,分析冬凌草中SSR位点信息,并利用Primer 3设计SSR引物,对其多态性进行初步评价。结果表明:研究共发现11 114个SSR,分布于8 873条独立基因(Unigenes)中,SSR位点出现频率为24.90%,共有55种重复基元,平均每3 517bp含1个SSR位点。SSR位点所包含的重复类型中,二核苷酸重复是主要类型,占总SSR的47.71%;其次是单碱基重复类型(35.46%)。SSR所包含的重复基元中,单核苷酸重复基元A/T和二核苷酸重复基元AG/CT是优势重复基元,分别占总SSR的34.95%、30.16%。多态性进行评价结果表明冬凌草转录组SSR类型丰富,多态性潜能高。Taking transcriptome of Rabdosia rubescens as research object, using Microsatellite （MISA） software, SSR loci information of Rabdosia rubescens was analyzed, and SSR primers were designed to evaluate the polymorphism. The results showed that 11 114 SSR loci were found,which distributed in 8 873 Unigenes, SSR loci frequency was 24. 90%. There were a total of 55 kinds of repeating units,average per 3 517 bp containing a SSR loci. The dinucleotide repeating was the most abundant type,accounting for 47. 71% of the total number of SSRs,followed by a single base repeat type （35. 46%）. A/T and AG/CT were the main repeat motifs,accounting for 34. 95% and 30. 16% ,respectively. Conclusion was SSR loci of Rabdosia rubescen had a high frequency, high polymorphism.

关 键 词：冬凌草 转录组 SSR 

分 类 号：S567.239[农业科学—中草药栽培]