纳洛酮预处理对吗啡抑制人乳腺癌MCF-7细胞生长增殖的影响  

The effects of naloxone pretreatment on morphine-induced inhibition of growth and proliferation of human breast cancer MCF-7 cells

作　　者：韦祎 覃怡[1] 利莉[1] 谢玉波[1] 

机构地区：[1]广西医科大学第一附属医院麻醉科,南宁市530021

出　　处：《临床麻醉学杂志》2016年第1期58-60,共3页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金(81160289);国家自然科学基金(81560500);广西科学研究与技术开发计划课题(桂科攻1355005-1-6);广西自然科学基金(2013GXNSFBA019156)

摘　　要：目的观察纳洛酮预处理对吗啡抑制人乳腺癌MCF-7细胞生长增殖的影响。方法人乳腺癌MCF-7细胞培养至对数生长期,采用随机数字表法,将其分为四组:空白对照组(C组),10μmol/L吗啡组(M组),10μmol/L纳洛酮组(N组)及10μmol/L吗啡+10μmol/L纳洛酮组(MN组)。M组在培养液中加入吗啡,使吗啡的终浓度为10μmol/L;N组在培养液中加入纳洛酮,使其终浓度为10μmol/L;MN组则先在培养液中加入纳洛酮,使其浓度为10μmol/L,孵育30min后再将吗啡加入培养液中,使其终浓度为10μmol/L;对照组不加入任何药物。采用四唑盐(MTT)法和克隆形成实验观察细胞生长增殖的变化,流式细胞仪检测细胞周期分布和细胞凋亡率。结果C组与N组细胞活力、克隆形成率、细胞周期分布及细胞凋亡率差异无统计学意义;M组及MN组细胞的生长速度明显慢于C组,克隆形成率、细胞停滞在S期的比例明显低于C组,而细胞凋亡率、细胞停滞在G2/M期的比例明显高于C组(P<0.05);M组与MN组细胞活力、克隆形成率、细胞周期分布及细胞凋亡率差异无统计学意义。结论在吗啡抑制人乳腺癌MCF-7细胞生长增殖、促进细胞凋亡的过程中,纳洛酮没有发挥拮抗作用,这说明吗啡抑制乳腺癌MCF-7细胞生长增殖的作用与阿片受体途径无关。Objective To observe the effects of naloxone pretreatment on morphine-induced inhibition of growth and proliferation of human breast cancer MCF-7 cells. Methods Human breast cancer cells MCF-7 were randomly divided into 4 groups. 10 μmol/L morphine group （group M）, 10 vmol/L naloxone group （group N）, 10μmol/L morphine＋10 μmol/L naloxone group （group MN） and control group （group C）. MCF-7 cells were cultured to logarithmic phase. The cells in group M were incubated with 10 μmol/L morphine while the cells in group N were incubated with 10μmol/L naloxone. The cells in group MN were incubated with 10 vmol/L naloxone for 30 min and then 10 μmol/L morphine was added to the culture medium. The cells in group C were incubated with normal culture medium. The viability of cells was determined by MTT assay and the proliferation of cells was determined by colony formation assay. The cell cycle progression and apoptosis were assessed by flow cytometry.Results No significant changes were found in the viability, colony formation, cell cycle progression and apoptotic rate between group C and group N. Compared with group C, the viability and clones of MCF-7 cells, the proportion in S phase were decreased while the proportion in G2/M phase and the apoptotic rate of cells were increased in group M and group MN （P〈0. 05）. There were no difference in the viability, rate of colony formation, cell cycle progression and apoptotic rate of cells between group M and group MN. Conclusion Naloxone could not antagonize the anti-growth effects of morphine on MCF-7 cells. Opioid receptors may not be involved in morphine-induced inhibition of growth and proliferation of human breast cancer MCF-7 cells in vitro.

关 键 词：吗啡 MCF-7细胞 增殖 纳洛酮 凋亡 

分 类 号：R614[医药卫生—麻醉学] R737.9[医药卫生—外科学]