姜黄素对海水吸入性肺损伤的防治作用  

Effects of curcumin on the sea water drowning induced lung injury

作　　者：谭远辉[1] 刘健[1] 朱四海[1] 童建华[1] 纪木火[1] 李伟彦[1] 

机构地区：[1]南京大学医学院临床学院南京军区南京总医院麻醉科,210002

出　　处：《临床麻醉学杂志》2016年第1期61-64,共4页Journal of Clinical Anesthesiology

基　　金：南京军区南京总医院重点课题(YYZD2014001)

摘　　要：目的探讨姜黄素对海水吸入性肺损伤的防治作用及机制。方法 120只雄性SD大鼠随机均分为五组：空白对照组（C组）、生理盐水组（N组）、海水组（S组）、高剂量姜黄素100mg/kg组（CH组）、低剂量姜黄素20mg/kg组（CL组）。S组、CH组和CL组建立海水吸入模型。吸入海水前2d,姜黄素组每天腹腔注射姜黄素100或20mg/kg,2次/天,C、N和S组腹腔注射等容PBS溶液。测定吸入海水前（基础值,T1）、吸入海水后15 min（T2）、30 min（T3）、1h（T4）、4h（T5）、24h（T6）时PaO2、PaCO2、右肺中叶湿干重比（W/D）。T3时测定左肺下叶丙二醛（MDA）和超氧化物歧化酶（SOD）的浓度,右肺下叶组织行常规病理切片检查。结果 T2时N组,T2~T4时S、CH和CL组PaO2明显低于C组（P〈0.05）;T2~T4时S、CH和CL组PaCO2明显高于C组（P〈0.05）。T2~T4时N组PaO2明显高于,PaCO2明显低于S组（P〈0.01）。T2、T3时CH组PaO2明显高于S组（P〈0.05）。S、CH和CL组MDA明显高于C组（P〈0.05）;N组和CH组MDA明显低于,SOD明显高于S组（P〈0.05）。T2~T4时S、CH和CL组肺组织W/D明显高于C组（P〈0.05）;T2~T4时CH组和N组肺组织W/D明显低于S组（P〈0.05）。海水吸入后S组出现明显的肺泡破裂,肺泡、肺间质水肿,炎细胞浸润,CH组能明显减轻肺组织损伤及炎性细胞浸润,但CL组与S组差异无统计学意义。结论预防性使用姜黄素100 mg/kg能减轻海水（4 ml/kg）吸入导致的早期（1h内）肺损伤,其机制可能与其减轻肺组织的氧化应激有关。Objective To observe the effects and possible mechanisms of eurcumin on the lung injury induced by sea water drowning.Methods One hundred and twenty male SD rats were randomly divided into 5 groups： control group （group C）, normal saline group （group N）, sea water group （group S）, high dose of curcumin group （100 mg/kg, group CH） and low dose curcumin group（20 mg/kg,group CL）. The model of sea water drowning was established in group S, group CH and group CL. Two days before sea water drowning, curcumin was used by intraperitoneal injection 100 or 20 mg/kg, twice a day. Constant volume of PBS was used by intraperitoneal injection in other three group. The arterial PaO2, PaCO2 and the wet-to-dry weight ratio（W/D） of. the middle lobe of fight lung were measured before drowning and 15 min, 30 rain, 1 h, 4 h and 24 h after drowning. At 30 min after drowing, the concentration of MDA and SOD of the inferior lobe of left htng were assayed and pathological changes of the inferior lobe of fight lung were examined by HE-staining.Results PaOz at T2 in group N and atT2- T4 in group S, CH, CL were significantly lower than in group C（P〈0. 05）. PaCO2 at T2-T4 in group S, CH, CL were significantly higher than in group C（P〈0. 05）. PaO2 were significantly higher and PaCO2 were significantly lower at T2-T4 in group N than in group S（P〈0.01）. PaO2 at T2 ,T3 in group CH were significantly higher than in group S（P〈0.05）. MDA in group S, CH, CL were significantly higher than in group C （P （0. 05 ）. MDA were significantly lower and SOD were significantly higher in group N and group CH than in group S（P〈0.05）. The W/D at T2-T4 in group S, CH, CL were significantly higher than in group C（P〈0. 05）. The W/D in group CH and group N at T2-T4 were significantly lower than in group S（P〈0. 05）. Animal in group S showed alveolar rupture, alveolar and pulmonary interstitial edema, and inflammatory cell infiltration severely. Only animal in group CH could significantly attenua

关 键 词：姜黄素 海水吸入 肺损伤 氧化应激 

分 类 号：R285.5[医药卫生—中药学]