检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:雷娅红 况卫刚 郑春生[3] 汪万春[3] 李春杰[1] 高文娜[3]
机构地区:[1]草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州730020 [2]中国农业大学农学与生物技术学院,北京100193 [3]北京出入境检验检疫局检验检疫技术中心,北京101312
出 处:《草业科学》2016年第1期46-53,共8页Pratacultural Science
基 金:国家质量监督检验检疫总局科技计划项目--进境牧草种子真菌筛查技术研究初探(2015IK225);国家质量监督检验检疫总局科技计划项目"进境种子携带植物病原细菌筛查技术初探"(2014IK005);国家质量监督检验检疫总局公益性课题--应用Si RNA测序技术筛查重要检疫性植物病毒方法研究(201310068);进境牧草种子有害生物风险评估(2015BK024)
摘 要:采用平皿测定法对我国北京口岸2014年进境草种高羊茅(Festuca arundinacea)、多年生黑麦草(Lolium perenne)和苏丹草(Sorghum sudanense)携带真菌进行分离培养,利用ITS4和ITS5引物对其内转录间隔区(Internal Transcribed Spacer,ITS)进行扩增,PCR产物纯化后测序并与数据库中的已知序列进行BLAST比对,对种带真菌进行初步鉴定。研究结果表明,不同来源种子携带真菌种类差异较大,从5个国家的7个进境草种批次中共检测出13属21种48株真菌,主要菌群为镰刀菌属(Fusarium spp.)、曲霉属(Aspergillus spp.)、茎点霉属(Phoma spp.)和链格孢属(Alternaria spp.)。基于ITS序列构建系统发育树,能够将所有分离到的真菌鉴定到属,但是在种水平上鉴定有限,不能将细极链格孢(Alternaria tenuissima)、细交链孢(A.alternata)和乔木链格孢(A.arborescens)很好地区分开。本研究明确了5个来样量较大国家的草种带菌情况,ITS基因能够运用于口岸草种携带真菌的初步检测鉴定。The 7 grass seed samples imported from 5 countries were analyzed for seed-borne fungi by Petri-dish testing.The samples included perennial ryegrass( Lolium perenne),tall fescue( Festuca arundinacea) and sudan grass( Sorghum sudanense) in 2014. The ITS( Internal transcribed spacer) segment was amplified using ITS4 and ITS5 as primers,then purified and sequenced. The ITS sequences obtained were blasted with known ITS sequences in database. The results showed that 48 fungal strains belonged to 21 species of 13 genera were isolated,and the seed-borne fungal species of seed samples were significantly different from each other. The fungal species were mainly Fusarium spp. Aspergillus spp.,Phoma spp.,and Alternaria spp.. According to the ITS phylogenetic tree,all fungi could be identified to genus,which were hard to species. Alternaria tenuissima,A. alternata,and A. arborescens couldn't be separated. The study analyzed seed-borne fungi of imported grass seeds from 5 countries,and the ITS sequences were applied for seedborne fungi of imported grass seeds preliminary detection and identification.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.70