基于RNA-Seq技术的苜蓿根蘖性状发生相关下调基因  

The down regulated genes related with creeping-rooted characteristics of alfalfa based on the RNA-seq

作　　者：郭云[1] 王铁梅[1,2] 

机构地区：[1]北京林业大学林学院,北京100083 [2]北京林业大学草地资源与生态研究中心,北京100083

出　　处：《草业科学》2016年第1期75-85,共11页Pratacultural Science

基　　金：国家自然科学基金--基于RNA-seq技术的苜蓿根蘖性状发生分子机制研究(31201843);北京林业大学校级大学生创新项目--基于RNA-seq技术的苜蓿根蘖性状发生分子机制的研究(X201410022009)

摘　　要：为了从分子水平上分析苜蓿(Medicago sativa)根蘖性状的发生机制,对根蘖型苜蓿的根蘖与非根蘖根部进行RNA-seq转录组文库构建,并分析差异表达基因及其功能。分别提取品系"BL-101"的根蘖型苜蓿根蘖与非根蘖部位总RNA,利用磁珠法分离m RNA后通过PCR扩增构建RNA-Seq转录组文库,并对差异表达基因进行分析,得到差异表达基因15 978条,本研究重点分析下调基因,通过生物过程分类,发现与根蘖性状发生有关的表达下调的基因为5个,经生物学信息分析确定,其中2个属于Lon蛋白酶家族,其余分别属于植物肌动蛋白酶家族,硫氧还蛋白和脂氧合酶,其与植物抗逆性相关,且与植物激素ABA的调控有关。研究结果可以为探讨苜蓿根蘖性状发生的分子机制提供理论依据。In order to understand the mechanism of alfalfa creeping-rooted characteristics at the molecular level,the transcriptome library of non creeping-rooted parts and the creeping-rooted parts of alfalfa variety ＂BL-101＂with creeping-rooted characteristics were constructed and the differentially expressed genes and their functions were analyzed. The results showed that there were totally 15 978 differentially expressed genes,including five down regulated genes involved in the biological rooting processes. For these 5 genes,2 genes belonged to Lon protease family which associated with the regulation of plant hormone ABA and the other 3 genes belong to plant actin protease family,thioredoxin and lipoxygenase,respectively. These results could provid the theory basis to understand the molecular mechanism of creeping-rooted characteristics.

关 键 词：苜蓿 根系生长 生物信息学 脱落酸 

分 类 号：S541.9[农业科学—作物学]