草地早熟禾愈伤组织对NaCl胁迫的生理响应  

Physiological response of Kentucky bluegrass calli under NaCl treatment

作　　者：徐海鹏[1] 李慧萍[1] 金小煜 金宁[2] 牛奎举 马晖玲[1] 

机构地区：[1]甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃农业大学园艺学院,甘肃兰州730070

出　　处：《草业科学》2016年第1期86-92,共7页Pratacultural Science

基　　金：甘肃农业大学国家级大学生创新创业训练计划项目(201510733001);甘肃农业大学"大学生科研训练计划(SRTP)项目"(20150225);国家自然科学基金"草地早熟禾种间体细胞杂交的研究"(31160482)

摘　　要：以草地早熟禾(Poa pratensis)午夜Ⅱ号愈伤组织为材料,研究NaCl胁迫对其生长、细胞膜相对透性、游离脯氨酸、丙二醛、可溶性蛋白及抗氧化酶活性的影响。结果表明,低浓度NaCl胁迫对午夜Ⅱ号愈伤组织的生长有促进作用,高浓度NaCl胁迫对愈伤组织生长具有抑制作用,且当NaCl浓度高于1.5%时愈伤组织开始出现褐化的现象;随NaCl浓度的升高,其胁迫程度也随之加强,丙二醛(MDA)和可溶性蛋白含量呈现先升高后降低的趋势,游离脯氨酸(Pro)含量和细胞膜相对透性则呈现出一直增加的趋势;过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性随NaCl胁迫程度的增加呈先升高后降低的趋势,在NaCl浓度为1.5%时酶活性达到最高。研究结果为草地早熟禾愈伤组织耐盐突变体的筛选奠定了基础。The effects of Na Cl treatment on calli generated from Kentucky bluegrass cultivars Midnight Ⅱ were investigated by measuring calli growth,relative membrane permeability,contents of proline,MDA,and protein and the activities of antioxidant enzymes. The results showed that the calli growth of Midnight Ⅱ was stimulated under low concentration of Na Cl whereas it was inhibited under high concentration of Na Cl and the browning was observed if Na Cl concentration was higher than 1. 5%. With the increase of Na Cl concentration,MDA and protein content increased firstly and then decreased. However,relative membrane permeability and proline content always increased. The activities of POD,SOD and CAT also increased firstly and then decreased with the increase of Na Cl concentration which reached the peaks with the concentrations of 1. 5% Na Cl. The present study provided useful knowledge for screening salt resistant mutants of kentucky bluegrass.

关 键 词：NACL胁迫 草地早熟禾 愈伤组织 生长 生理生化 

分 类 号：S688.4[农业科学—观赏园艺]