肠胃清含药血清对人结肠癌HCT116/L-OHP耐药细胞凋亡作用的影响  

Research on Changwei Qing medicated serum increasing chemosensitivity towards human colon cancer resistant cells HCT116 / L-OHP

作　　者：肖海娟[1] 孙珏[2] 许建华[2] 范忠泽[2] 

机构地区：[1]陕西中医药大学附属医院肿瘤科,陕西咸阳712000 [2]上海中医药大学附属普陀医院肿瘤科,上海200062

出　　处：《上海中医药杂志》2016年第2期79-83,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81303135);陕西省科技厅自然科学基金青年人才项目(2015JQ8288)

摘　　要：目的探讨中药复方肠胃清(CWQ)逆转人结肠癌HCT116/L-OHP细胞耐药的分子机制。方法提取肠胃清含药血清,设置不同浓度,采用MTT法确定肠胃清的最大无毒剂量和逆转指数(RI);采用彗星实验法检测肠胃清联合奥沙利铂(L-OHP)对耐药细胞DNA损伤的影响,流式细胞术观察肠胃清联合奥沙利铂(L-OHP)对耐药细胞凋亡的影响。结果肠胃清含药血清可以逆转人结肠癌HCT116/L-OHP耐药细胞对L-OHP的耐药性,逆转指数(RI)为2.63倍。肠胃清联合L-OHP能促进耐药细胞发生DNA损伤(P<0.001)及凋亡(P<0.01)。结论肠胃清能够逆转人结肠癌铂类化疗耐药,其机制与促进肿瘤细胞DNA损伤、诱导凋亡有关。Objective To investigate the molecular mechanisms of resistance of traditional Chinese medicine Changwei Qing（ CWQ） reversal of human colon cancer oxaliplatin. Methods Extract CWQ containing serum,using MTT assay to detect CWQ reversal index（ RI）,using the comet assay to detect cell DNA damaged and detect cell apopotosis by flow cytometr in resistant cells. Results CWQ drug serum containing reversing HCT116 / L-OHP resistant cells to L-OHP resistance reversal index（ RI） was 2. 63 times. The CWQ L-OHP group of resistant cells could promote DNA damage（ P 0. 001） and apoptosis（ P 0. 01）. Conclusion CWQ can reversal drug resistance of human colon cancer to Platinum-based Chemotherapy,and it＇s mechanism may has relationship with promotion DNA damage and apoptosis occurred in cancer cells.

关 键 词：肠胃清 奥沙利铂 耐药 结肠癌 凋亡 

分 类 号：R285[医药卫生—中药学]