胶束增敏荧光法测定大鼠血清中荜茇宁  

Determination of Piperlonguminine in Rat Serum Using Micelle Sensitized Fluorescence Spectrometry

作　　者：领小[1] 苗澍[1] 敖登高娃[1] 

机构地区：[1]内蒙古大学化学化工学院,内蒙古呼和浩特010021

出　　处：《分析测试学报》2016年第2期225-228,234,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(81060366);内蒙古自然科学基金项目(2014MS8024)

摘　　要：蒙药荜茇有效成分荜茇宁具有荧光,且荧光可被表面活性剂吐温-20增敏,同时,其同步荧光光谱能消除血清样品的背景荧光对荜茇宁荧光光谱的干扰,据此建立了直接测定大鼠血清中荜茇宁浓度的新方法。荜茇宁的线性范围为5.47×10^(-3)~3.28μg·m L^(-1),检出限为1.20×10^(-3)μg·m L^(-1),样品回收率为100.6%,相对标准偏差为2.7%。方法可用于大鼠血清样品中荜茇宁浓度的直接测定。Piperlonguminine,the effective composition of the Mongolian medicine Piper longum L.,has fluorescence and its fluorescence signal could be greatly enhanced by the surfactant Tween- 20.The synchronous fluorescence spectroscopic method could eliminate the interference of the background fluorescence from serum samples on fluorescence spectra of piperlonguminine. According to this technique,a method was established for the determination of piperlonguminine in rat serum. The linear range of this method was in the range of 5. 47 × 10^（-3）- 3. 28 μg·m L- 1,the detection limit for piperlonguminine was 1. 20 × 10^（-3）μg·m L^（-1）,and the relative standard deviation（ RSD） was 2. 7%.The method was successfully applied in the detection of piperlonguminine in rat serum amples,with recovery of 100. 6%.

关 键 词：荜茇宁 胶束体系 荧光分析法 血清 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]