桉树叶挥发油的GC-MS分析研究  

Analysis of the Essential Oils from the Leaf of Eucalyptus by Gas Chromatography- Mass Spectrometry

作　　者：梁雯华 李先文[1] 

机构地区：[1]广东海洋大学理学院,广东湛江524088

出　　处：《安康学院学报》2016年第1期99-102,共4页Journal of Ankang University

基　　金：广东海洋大学大学生创新计划项目(1056613031)

摘　　要：桉树为桃金娘科桉属密荫大乔木,桉树叶具有消毒、杀菌止痛、护肤等功效。为进一步系统地研究桉树叶的有效成分以及更好地利用桉树资源,本实验采用经典的水蒸气蒸馏提取方法提取新鲜、自然晾干、低温50℃烘干三种不同前处理情况下的尾叶桉树叶的挥发油,通过运用气质联用方法对新鲜叶以及烘干叶挥发油的成分进行化学成分的种类以及含量分析。结果显示,自然晾干桉树叶出油率>低温50℃烘干桉树叶出油率>新鲜桉树叶出油率,并且新鲜叶以及低温50℃烘干叶挥发油的成分基本相同,含量基本相同,个别有差异。从样品1(新鲜)和从样品3(低温50℃烘干)挥发油中分离鉴定都得到40个峰,检测出36种化合物,所鉴定的样品1以及样品3的挥发油成分的相对质量分数,通过使用面积归一法得到的检测结果分别占总面积的99.82%和99.78%。挥发油主要成分为:1,8-桉叶素(42.9%/43.34%);α-蒎烯(32.39%/22.84%);右旋柠檬烯(6.65%/17.14%);乙酸松油酯(4.22%/4.86%);β-蒎烯(3.13%/1.96%);α-松油醇(2.11%/1.92%);蓝桉醇(1.27%/1.63%)。Eucalyptus is the eucalyptus dense shade trees of myrtle.Eucalyptusleavesaretheleavesofeucalyptus,with caring skin,relieving pain,sterilization and disinfection efficacy.For further systematically to study the effective components of eucalyptus leaves,and better use of eucalyptus resources,this experiment adopts the classic water vapor distillation extraction method to extract the fresh,natural drying and low temperature of 50℃ drying eucalyptus leaf essential oil,and using GC- MS analysis of two kinds of volatile oil composition.According to the results：natural dry eucalyptus leaves oil yield low temperature 50℃ dried eucalyptus leaves oil yield fresh eucalyptus leaves oil yield and two methods to extract the volatile oil components of eucalyptus leaves are basically the same.The content is basically the same and individual differences.From the sample 1（fresh） and 3（low temperature of 50℃ drying were separated to identify 40 peak and 36 compounds were detected.Measured by the area normalization method elements and identified by the relative mass fraction,accounted for 99.82%and 99.78%of total peak area.The main components of the volatile oil：Eucalyptol（42.9%/ 43.34%）;α- Pinene（32.39%/ 22.84%）;D- Limonene（6.65%/ 17.14%）;Terpinyl acetate（4.22%/4.86%）;β- Pinene（3.13%/1.96%）;α-Terpined（2.11%/1.92%）;Globulol（1.27%/1.63%）.

关 键 词：桉树叶 挥发油 GC-MS 

分 类 号：R284[医药卫生—中药学]