朊病毒病生物标记microRNA-142-3p可视化快速检测  

Visualized Detection of Prion Disease of Biomarker MicroRNA-14-3p

作　　者：张腾龙[1,2] 薄乐[1,2] 英那 郝文丽[1] 武智勇[1] 王雄[1] 孟轲音[2] 万家余[2] 陈志宝[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]中国人民解放军军事医学科学院军事兽医研究所

出　　处：《黑龙江八一农垦大学学报》2016年第1期64-67,共4页journal of heilongjiang bayi agricultural university

基　　金：国家自然科学基金(3120197);国家自然科学基金(3120114)

摘　　要：为了更快捷、方便的检测朊病毒病,根据已有报道确定micro RNA-144-3p作为朊病毒病的生物标记,通过mi RBase数据库公布的micro RNA-142-3p的核酸序列,设计特异性标记有生物素的俘获探针和标记有FAM的检测探针,从而建立了纳米金偶联核酸试纸条的高效,快捷,可视化检测方法。结果表明:该检测方法具有良好的特异性和较高的灵敏度(0.005 nmol·L-1)。该检测试纸条的建立在朊病毒病预防,控制,诊断过程中具有一定实际意义。For the purpose of detecting prion disease more rapidly and conveniently, microRNA-144-3p has been considered as a biomarker for prlon disease according to the pre-report. Based on miRBse sequence, the specific capture probe with marked biotin and detecting probe with marked FAM were designed in order to establish the high efficiency, rapid and visualized detection method, which coupled with nano-gold and nucleic indicator paper. Therefore, this method made a good foundation to prevent, control, diagnose prion diseases.

关 键 词：朊病毒 microRNA-142-3p 生物标记 可视化 检测 

分 类 号：S865.13[农业科学—野生动物驯养]