整合素连接激酶修饰的骨髓间充质干细胞改善大鼠梗死后心脏心功能  

Effect of Mesenchymal Stem Cells Transfected with Intergrin-Linked Kinase Gene on Myocardial Function

作　　者：王林[1,2] 童随阳 刘浙波 付金蓉[1] 曾彬[1] 夏豪[1] 

机构地区：[1]武汉大学人民医院心血管内科,湖北武汉430060 [2]十堰市郧阳区人民医院心血管内科,湖北十堰442500

出　　处：《武汉大学学报（医学版）》2016年第2期173-176,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金青年项目(编号:30900609);国家自然科学基金面上项目(编号:81270271;81570333);湖北省自然科学基金项目(编号:2015CFB684)

摘　　要：目的:探讨整合素连接激酶(ILK)修饰的骨髓间充质干细胞(MSCs)对心肌梗死后心脏心功能的影响。方法:取大鼠骨髓体外分离扩增培养MSCs,分别用ILK、ILK-siRNA及对照GFP腺病毒转染MSCs。结扎大鼠心脏左前降支1h后,分别将GFP-MSCs、ILK-MSCs、ILK-siRNA-MSCs多点注射到大鼠心肌梗死区周边,对照组注射等量PBS。结果:细胞移植4d后与GFP-MSCs移植组相比,ILK-MSCs组存活细胞数目明显增多,而ILKsiRNA-MSCs移植组存活细胞明显减少(P<0.05)。细胞移植3周后与GFP-MSCs移植组相比,ILK-MSCs组新生血管数目明显增多,且胶原蛋白沉积减少,梗死面积明显缩小,心功能各项指标明显改善(P<0.05);而ILK-siRNA-MSCs移植组新生血管数目明显减少,胶原蛋白沉积增多,梗死面积明显增加,心功能各项指标明显恶化(P<0.05)。结论:ILK增加MSCs存活率、促进梗死后心脏血管新生,从而抑制心室重构,改善心功能。Objective： To investigate whether intergrin-linked kinase （ILK） transduced by MSCs can im- prove heart function after heart infraction. Methods： MSCs were acquired from the bone marrow of adult rats. The ceils were isolated, purified, cultured, and transfeeted with Adv-ILK, Adv- ILK-siRNA, and Adv-GFP respectively. 1 × 106 ILK-MSCs, GFP-MSCs, ILK-siRNA-MSCs or PBS was respectively injected into rat hearts 1 h intramyoeardially after myocardial infarction. Results： It was observed that ILK-overexpressed MSCs increased MSCs survival at 4 the end of days and angiogenesis at the end of 3 weeks after transplantation into infraeted myocardium as compared with GFP-MSCs group and ILK-SiRNA-MSCs group. This enhanced response was as- sociated with attenuated left ventricular （LV） chamber dilation, reduced LV fibrosis, decreased infarct size and improved LV function. Conclusion： ILK modification with MSCs increases MSCs survival and angiogenesis, and preserves cardiac {unction and inhibits remodeling.

关 键 词：骨髓间充质干细胞 心肌梗死 整合素连接激酶 血管再生 生存 

分 类 号：R541.7[医药卫生—心血管疾病]