巨细胞病毒宫内感染对子代豚鼠海马神经元Tau蛋白磷酸化的影响  

Effect of Tau Protein Phosphorylation in the Descendant Guinea Pig with Cytomegalovirus Intrauterine Infection

作　　者：乔媛[1] 刘静[2] 肖娟[2] 刘涛[3] 李家福[1] 张元珍[1] 陈素华[2] 

机构地区：[1]武汉大学中南医院妇产科,湖北武汉430071 [2]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030 [3]山东省泰安市中心医院妇产科,山东泰安271000

出　　处：《武汉大学学报（医学版）》2016年第2期240-244,共5页Medical Journal of Wuhan University

基　　金：武汉大学青年教师资助项目(编号:2042015kf0154)

摘　　要：目的:研究巨细胞病毒(CMV)宫内感染对子代豚鼠海马神经元Tau蛋白磷酸化的影响。方法:建立豚鼠CMV宫内感染动物模型,PCR法筛选CMV DNA阴性豚鼠,雌雄同笼受孕;孕龄1-20d时,随机分成两组:感染组腹腔内接种CMV病毒悬液,对照组接种生理盐水,足月分娩的子代豚鼠喂养至3月龄后无菌采集脑组织,应用免疫组织化学法和免疫印迹法检测各组子代豚鼠海马神经元Tau、p-Tau(Ser 404)、PKA及PP2A蛋白表达水平。结果:与对照组相比,感染组子代豚鼠海马神经元Tau、p-Tau(Ser 404)及PKA蛋白的表达水平明显上升,而PP2A蛋白的表达水平明显下降,差异具有统计学意义(P<0.01)。结论:CMV宫内感染导致子代豚鼠海马神经元Tau蛋白磷酸化水平增加,可能与PKA及PP2A蛋白的表达异常相关。Objective： To study Tau protein phosphorylation of the descendant guinea pig with cytomeg- alovirus （CMV） intrauterine infection. Methods： The model of CMV intrauterine infection was built as follows： The female and male guinea pigs without CMV-infected identified by PCR were chosen and mated for pregnancy. The pregnant were randomly divided into two groups in gesta- tional age from one to twenty： CMV was injected into abdominal cavity in CMV-infected group, and saline was injected in control group. These full term offspring were fed for 3 months to col- lect brain tissue in sterile conditions. The protein expression of Tau,p-Tau（Ser 404）,PKA, and PP2A were measured by immunohistochemistry and Western blotting. Results： Compared with those in control group, the protein expressions of Tau,p-Tau（Ser 404） ,and PKA in CMV-infec- ted group significantly increased, while the protein expression of PP2A significantly decreased （P 〈0.01）. Conclusion： CMV intrauterine infection can increase Tau protein phosphorylation, and the abnormal expression of both PKA and PP2A might be involved in the process.

关 键 词：巨细胞病毒 宫内感染 豚鼠 TAU蛋白 磷酸化 

分 类 号：R714.53[医药卫生—妇产科学]