拟南芥SOAR1基因响应ABA与渗透胁迫的表达分析  

Expression Analysis of Arabidopsis SOAR1 in Response to ABA and Osmotic Stress

作　　者：姜上川[1] 梅超[1] 王小芳[1] 张大鹏[1] 

机构地区：[1]清华大学生命科学学院,北京100084

出　　处：《河南农业科学》2016年第2期29-34,48,共7页Journal of Henan Agricultural Sciences

基　　金：国家转基因作物品种培育科技重大专项(2014ZX08009003);国家基础研究重大专项(2012CB114300-002)

摘　　要：拟南芥PPR蛋白SOAR1是ABA信号转导的关键调节因子。为阐明SOAR1基因表达对ABA和渗透胁迫的响应,以及其对不同时期幼苗生长响应ABA的调控,通过拟南芥基因调控信息网站(AGRIS)分析了SOAR1基因上游可能的转录因子结合位点(顺式作用元件),并进行了不同时期幼苗受ABA诱导的生长抑制试验以及ABA处理、渗透胁迫条件下SOAR1基因的表达分析。结果表明,SOAR1启动子序列中存在多个潜在的应答ABA和逆境胁迫信号的顺式作用元件。SOAR1调控不同时期幼苗生长对ABA的敏感性,SOAR1表达调低的突变体soar1-2显著促进植物对ABA的敏感反应,而SOAR1过表达株系OE1则对ABA显著不敏感。基因表达分析结果显示,SOAR1表达量在低浓度ABA处理6 h后小幅度上调,高浓度ABA处理6 h后变化程度较低;而在低浓度ABA处理后萌发24 h的种子和生长7 d的幼苗中,SOAR1表达随ABA浓度增长而上调。在甘露醇和PEG-6000诱导的渗透胁迫处理后,SOAR1表达受到一定抑制。The Arabidopsis PPR（ pentatricopeptide repeats） protein SOAR1 is a crucial regulator of ABA signaling. In order to elucidate how SOAR1 expression responds to ABA and osmotic stress,and how SOAR1 regulates seedlings response to ABA during different post-germination growth stages,in this study,we used Arabidopsis Gene Regulatory Information Server（ AGRIS） to analyze the potential ABA and stress response elements in upstream region of SOAR1 gene,conducted the ABA-induced post-germination growth arrest assay in different growth stage,and analyzed SOAR1 expression level under ABA or osmotic treatment. The results showed that there were several potential ABA and stress response elements in the promoter region of SOAR1 gene. SOAR1 regulated the seedlings sensitivity to ABA in different post-germination growth stage,soar1-2 mutant with SOAR1 expression decreased showed promoted sensitivity to ABA,while SOAR1 overexpression line OE1 was significantly insensitive to ABA. The expresssion analysis showed that SOAR1 was slightly increased after 6 h treatment of low concentration ABA,but was not changed much after 6 h treatment of high concentration ABA; while in the germinating seeds（ 24 h） or seedlings（ 7 d） after treatment of low concentration ABA,SOAR1 was up-regulated with the increase ofABA concentration. Under the osmotic stresses induced by D-mannitol and PEG-6000,the expression level of SOAR1 was repressed.

关 键 词：脱落酸(ABA) SOAR1 幼苗生长 渗透胁迫 基因表达 

分 类 号：Q78[生物学—分子生物学]