热激对家蚕后部丝腺磷酸化蛋白质组的影响  

Effect of Heat Shock on Phosphorylation Proteins of Silkworm Posterior Silk Gland

作　　者：夏爱华[1,2] 李季生[1,2,3] 李娜[1,2] 贾漫丽[1,2] 王晖[1,2] 杨贵明[1,2] 

机构地区：[1]河北省高校特产蚕桑应用技术研发中心,河北承德067000 [2]承德医学院蚕业研究所,河北承德067000 [3]浙江大学动物科学学院,浙江杭州310029

出　　处：《河南农业科学》2016年第2期127-133,137,共8页Journal of Henan Agricultural Sciences

基　　金：河北省教育厅资助项目(Q2012026);承德医学院院级课题(201538)

摘　　要：为了探索蛋白质磷酸化在高温环境胁迫中发挥的作用,以秋丰×白玉家蚕为试验材料,将5龄第3天的家蚕放置在40℃培养箱内热激10 min,以未热激的家蚕为对照,利用双向电泳和质谱技术对后部丝腺磷酸化蛋白进行了分析和鉴定。结果发现,与对照相比,9个磷酸化蛋白在短期的热激之后表达量下降,包括腺苷酸激酶,延伸因子EF-1δ、EF-1β',核糖体蛋白P0、P1、P2以及轻链丝心蛋白等。GO分析表明,这些蛋白质主要参与能量代谢和丝心蛋白合成。对以上磷酸化蛋白的结构和功能进行分析,并利用Net Phos工具进行磷酸化位点预测,结果表明,这些蛋白质二级结构主要以α螺旋或无规则卷曲为主,无跨膜结构域,均存在磷酸化修饰。In order to preliminarily explore the function of protein phosphorylation under environmental stress from the molecular level,silkworm strain Qiufeng × Baiyu of 5th instar 3rd day were placed into incubator at 40 ℃ for 10 min. We analyzed and identified the differentially expressed phosphorylation proteins of silkworm utilizing two-dimensional electrophoresis and mass spectrometry. Results showed that nine protein spots related to silk synthesis,including adenosine kinase,elongation factor 1 delta,elongation factor 1 beta＇,ribosomal protein P0,ribosomal protein P1,ribosomal protein P2 and fibroin L-chain,etc.,showed down-regulated trends after heat shock. Functional analysis revealed that these proteins were involved in energy metabolism and fibroin synthesis. In addition,we further analyzed the structure and function of these phosphorylated proteins,and employed the Net Phos to predict their phosphorylation sites. Results showed that these proteins took alpha helix or random curl as their main secondary structure form,and did not possess transmembrane domain. Moreover,phosphorylated modification was found in all proteins.

关 键 词：热激 家蚕 后部丝腺 磷酸化蛋白 双向电泳 质谱 生物信息学 

分 类 号：S885[农业科学—特种经济动物饲养]