桃美人景天的组织培养与快速繁殖  

Tissue Culture and Rapid Propagation of Pachyphytum ‘Blue Haze'

作　　者：赵海乐[1] 孙国庆[1] 冯凯[2] 侯宇[2] 温钢[1] 

机构地区：[1]吉林化工学院生物与食品工程学院 [2]北华大学化学与生物学院,吉林吉林132022

出　　处：《吉林化工学院学报》2016年第1期54-60,共7页Journal of Jilin Institute of Chemical Technology

摘　　要：以景天科植物桃美人（Pachyphytum‘Blue Haze’）的叶片作外植体进行组织培养,研究不同激素配比对组织培养的诱导、分化、生根的影响,以建立其快速繁殖体系.结果表明,叶片最适灭菌方式：75%酒精30 s＋无菌水冲洗＋0.1%Hg Cl2溶液消毒5 min＋无菌水漂洗4~5次;愈伤组织诱导最佳培养基：MS＋1.0 mg/L NAA＋1.5 mg/L 6-BA;不定芽诱导的最佳培养基为MS＋3.0 mg/L 6-BA,诱导率可达76.5%;生根培养以1/2MS＋3.0 mg/L 6-BA为最好,其生根率可达99%,而且幼苗长势旺盛,能够满足快速繁殖的要求.In order to establish a rapid propagation system for Pachyphytum ＇Blue Haze＇,the effect of different hormones proportion on callus adventitious buds induction,differentiation,rooting of Pachyphytum ＇Blue Haze＇on leaf as ex-plants respectively was studied. The results show that the optimal sterilization of leaves： 75%alcohol 30 s ＋ sterile ducks and drakes ＋ 0. 1% Hg Cl2 disinfection 5min ＋ sterile water rinse Wash 4 ~ 5times; The best medium was MS ＋ 1. 0 mg / L NAA ＋ 1. 5 mg / L 6-BA; The best medium was MS ＋ 3. 0 mg / L 6-BA for adventitious buds induction,the induction rate was up to 76. 5%; The best medium of rooting medium was 1 /2MS ＋ 3. 0 mg / L 6-BA,the rooting rate was 99% and the seeding growth vigour exuberantly. The seeding could meet the requirement of rapid propagation production.

关 键 词：红化妆景天 组织培养 叶片 愈伤组织 

分 类 号：Q945.5[生物学—植物学]