蓬子菜中活性成分DXG大鼠血浆蛋白结合率测定  

Determination of plasma protein binding rate of DXG from Galium verum L. in rat

作　　者：崔明宇[1] 孙志伟[2] 李艳凤[1] 马英丽[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]哈尔滨商业大学药学院,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2015年第6期678-681,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金项目(201281274034);黑龙江省教育厅科学技术研究项目(12531625)

摘　　要：考察蓬子菜中活性成分DXG在大鼠血浆中的蛋白结合情况.采用平衡透析法测定DXG的大鼠血浆蛋白结合率.建立DXG透析外液和透析内液的HPLC测定方法,求得高、中、低不同质量浓度下,DXG的血浆蛋白结合率分别为29.39%,29.85%,32.94.该方法专属性强,灵敏度高,符合生物样品测定要求要求.DXG的大鼠血浆蛋白结合率较低,且不具有质量浓度依赖性.To determinate the protein - bound fraction of serum of DXG from Galium verum L. Equilibrium dialysis was used to determine the in vitro binding rate of DXG to rat plasma. A HPLC method for determination of DXG dialysated inside solution and outer solution was established. Plasma protein binding rate of DXG in different concentration was 29, 39%, 29.85%, 32.94%, respectively. The methods were specific and sensitive, which can be used in determination of biological specimen. The plasma protein binding of DXG in rats re- veals a low binding and has no concentration dependence.

关 键 词：蓬子菜 DXG 血浆蛋白结合率 平衡透析法 

分 类 号：R96[医药卫生—药理学]