饲料中添加龙须菜对眼斑拟石首鱼生长、脂肪酸组成、免疫及肠道的影响  

Effect of dietary Gracilaria lemaneiformis on growth performance,fatty acid composition,immunity and intestinal histology of red drum( Sciaenops ocellatus)

作　　者：李雅婷[1] 陈明[1] 曾帅霖 刘永坚[1] 田丽霞[1] 

机构地区：[1]中山大学生命科学大学院水生经济动物研究所,广东广州510275

出　　处：《南方水产科学》2016年第1期85-93,共9页South China Fisheries Science

基　　金：国家科技支撑计划项目(2012BAC07B05)

摘　　要：为了研究添加龙须菜（Gracilaria lemaneiformis）对眼斑拟石首鱼（Sciaenops ocellatus）生长、脂肪酸组成、免疫和肠道的影响,在饲料中分别添加0（对照组）、1%、2%、3%、4%和5%的龙须菜,饲养初始体质量（9.38±0.06）g的眼斑拟石首鱼8周。结果表明,添加龙须菜对鱼体成活率、增重率（WG）和特定生长率（SGR）均不存在显著性影响（P〉0.05）,但1%~4%组的增重率均高于对照组,1%组的饲料效率和特定生长率最高;所有龙须菜添加组全鱼脂肪酸中的二十二碳六烯酸（DHA）均高于对照组,且添加量为5%时DHA显著高于对照组（P〈0.05）;肝脏中超氧化物歧化酶（SOD）活性在4%组显著高于对照组（P〈0.05）;3%组肠道黏膜褶最高且组织状况最好。由此可见,饲料中添加龙须菜对眼斑拟石首鱼生长有一定的促进作用,且添加适量龙须菜可以改善眼斑拟石首鱼的肝脏和肠道健康,提高全鱼DHA含量。We investigated the effect of dietary graded level of Gracilaria lemaneiformis on the growth performance,fatty acid composition,body composition,immunity and intestinal histology of red drum（ Sciaenops ocellatus） [initial weight（ 9. 38 ± 0. 06） g]. Six isoproteic and isolipidic diets（ crude protein： 41%; crude fat： 10%） were formulated containing 0%,1%,2%,3%,4% and5% G. lemaneiformis. After eight weeks of feeding,no significant difference was found in survival,weigh gain（ WG） and specific growth ratio（ SGR）（ P〉0. 05）,but weight gains in 1% ~ 4% groups were higher than that in the control whereas 1% group had the highest feed efficiency and SGR among all treatments. The concentrations of docosahexaenoic acid（ DHA） were significantly higher in5% group than that in the control（ P〈0. 05）. Meanwhile,the liver superoxide dismutase（ SOD） in 4% group was significantly higher than that in the control（ P〈0. 05）; well-developed intestinal villi was presented in 3% addition group. The results indicate that dietary supplementation of G. lemaneiformis can improve the growth as well as liver and intestinal health,increasing whole fish DHA content.

关 键 词：龙须菜 眼斑拟石首鱼 脂肪酸 免疫 肠道 

分 类 号：S963.7[农业科学—水产养殖]