益气复生方联合5-Fu对人结肠癌多药耐药裸鼠皮下移植瘤的抑制作用  

Inhibitory Effect of “Yiqi Fusheng Decoction” Combined with 5-Fu on the Xenografts in Human Colorectal MDR Carcinoma Nude Mice

作　　者：隋华[1] 张彦博[1] 周利红[1] 朱蕾[2] 郑萍[3] 温昊天 李琦[1] 邓皖利[1] 

机构地区：[1]上海中医药大学附属曙光医院肿瘤科,上海201203 [2]新疆维吾尔自治区昌吉州人民医院,新疆昌吉831100 [3]新疆医科大学中医学院,新疆乌鲁木齐830000 [4]上海中医药大学,上海201203

出　　处：《上海中医药大学学报》2016年第1期45-49,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81460704;81573805);新疆维吾尔自治区科技援疆计划项目(201291169)

摘　　要：目的:探讨益气复生方联合5-氟脲嘧啶(5-Fu)对裸鼠移植性大肠癌实体瘤的抑制作用。方法:建立裸鼠人结肠癌皮下移植瘤耐药模型,随机分为模型组、阳性对照组(5-Fu组)及益气复生方低、中、高剂量联合5-Fu组。分别给予相应药物连续干预28 d后,每组随机取部分裸鼠脱臼处死,剥取肿瘤,计算抑瘤率,并检测肿瘤细胞凋亡情况及肿瘤组织内5-Fu含量;其余裸鼠正常摄食饮水,观察其生存期。结果:益气复生方低、中、高剂量联合组的抑瘤率和中位生存时间均明显高于阳性对照组(P<0.05),且呈剂量依赖性,其中益气复生方高剂量联合组的抑瘤率与中位生存时间明显高于其他各组(P<0.05);益气复生方干预后,肿瘤组织内5-Fu含量明显升高、肿瘤细胞凋亡率增加,并且随着益气复生方浓度的增加而增加,具有剂量依赖性。结论:益气复生方联合5-Fu可抑制人结肠癌多药耐药细胞裸鼠皮下移植瘤的生长,延长裸鼠的中位生存时间,且随着剂量的增加其作用增强。Objective： To investigate the inhibition of＂Yiqi Fusheng Decoction＂（ YQFS） combined with 5-fluorouracil（ 5-Fu） on human colon cancer xenograft tumors. Methods： Nude mouse model of colorectal MDR cancer was established,and randomly divided into model group,5-Fu group and YQFS low,medium and high dose combined with 5-Fu groups. After 28 days treatment with medicine respectively,the mice in each group were chose randomly and sacrificed to measure tumor tissues sizes,inhibition rate,apoptosis and concentration of 5-Fu in tumor,the remained mice were given food and water normaly to observe the survival. Results： The inhibition rate of tumor growth and the median survival time in YQFS low,medium and high dose combined with 5-Fu groups were significantly higher than that in 5-Fu group（ P〈0. 05） in dose dependent manner,in which the inhibition rate of tumor growth and the median survival time in YQFS high dose combined with 5-Fu group significantly higher than that of the other groups（ P〈0. 05）. The concentration of 5-Fu and cell apoptosis were increased with a dose-dependent manner after the treatment of ＂Yiqi Fusheng Decoction ＂. Conclusion： YQFS combined with 5-Fu could inhibit the proliferation of nude mice model with human multidrug resistance（ MDR） colorectal carcinoma and prolong survival time in a dose-dependent manner.

关 键 词：益气复生方 5-氟脲嘧啶 结肠癌 裸鼠 耐药 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]