黄连-大黄-肉桂复方对肝癌细胞增殖及PTEN-PI3K-AKT/SGK1信号通路的影响  

Effect of Rhizoma Coptidis-Rheum Officinale-Cinnamon Compound on the Proliferation and PTEN-PI3K-AKT/SGK1 Pathway of Hepatocellular Carcinoma Cells

作　　者：刘小美[1] 潘志强[1] 方肇勤[1] 宋菊敏[1] 卢文丽[1] 粱超[1] 张园园[1] 

机构地区：[1]上海中医药大学基础医学院,上海201203

出　　处：《上海中医药大学学报》2016年第1期50-54,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81473562);国家自然科学基金青年基金项目(81503481)

摘　　要：目的:观察黄连-大黄-肉桂复方对肝癌细胞增殖的抑制作用,并探索其作用机制。方法:采用醇提法提取黄连-大黄-肉桂复方药液,观察药物作用24 h后SMMC-7721人肝癌细胞株细胞形态的改变;通过MTT法检测不同浓度药物作用24 h、48 h和72 h对肝癌细胞增殖的影响;采用RTq PCR法检测肝癌细胞PTEN、AKT和SGK1基因的mRNA表达。结果:较低浓度的黄连-大黄-肉桂复方作用24 h对肝癌细胞的形态影响较小,而浓度增至2.1 mg/ml可使肝癌细胞部分固缩,大多细胞凋亡,其对肝癌细胞的恶性增殖具有显著抑制作用,并存在时效和量效关系,0.5 mg/ml药物作用48 h即可致肝癌细胞增殖半数得到抑制;药物作用后AKT mRNA表达明显降低,PTEN mRNA表达上调、SGK1 mRNA表达降低。结论:黄连-大黄-肉桂复方能有效抑制肝癌细胞的恶性增殖,且具有时效和量效关系;其抑制作用可能与调节细胞PTEN-PI3K-AKT/SGK1信号转导通路有关。Objective： To observe the inhibiting effect of Rhizoma Coptidis-Rheum Officinale-Cinnamon Compound（ RRC） on the proliferation of hepatocellular carcinoma cells and to explore the action mechanism of the compound. Methods：RRC was extracted with alcohol. The morphology of SMMC-7721 cells after 24 hours treatment with RRC was observed and the inhibiting effect on the proliferation of SMMC-7721 cells after 24,48 and 72 hours treatment with different concentration RRC was detected with MTT method. The mRNA expression of PTEN,AKT and SGK1 was measured with RTq PCR method.Results： The hepatocellular carcinoma cells morphology scarcely changed when they were treated with lower concentration RRC,but the cells pyknosis and apoptosis appeared when the concentration of RRC increased to 2. 1mg / ml. RRC could significantly inhibit the malignant proliferation of hepatocellular carcinoma cells. This inhibiting effect existed time-effect and dose-effect relationship. About half hepatocellular carcinoma cells were inhibited when they treated with 0. 5mg / ml RRC for 48 hours. Meanwhile,RRC down-regulated AKT（ P〈0. 05） and SGK1 mRNA expression and up-regulated PTEN mRNA expression of hepatocellular carcinoma cells. Conclusion： RRC can effectively inhibit the proliferation of hepatocellular carcinoma cells and the inhibition appears time-effect and dose-effect relationship. The effect of RRC may has the relationship with regulating the PTEN-PI3K-AKT / SGK1 pathway.

关 键 词：黄连-大黄-肉桂复方 肝癌细胞 细胞增殖 PTEN-PI3K-AKT/SGK1信号通路 

分 类 号：R285[医药卫生—中药学]