神经干细胞对创伤性脑损伤的修复作用  

Repair Effect of Neural Stem Cells Transplantation after Traumatic Brain Injury

作　　者：刘吉星[1] 侯博儒[1] 杨文桢[1] 李小会[1] 马浚宁 陈四化[1] 严贵忠 尹立山[1] 王刚[1] 任海军[1] 

机构地区：[1]兰州大学第二医院神经外科,兰州730030

出　　处：《世界科技研究与发展》2016年第1期136-142,共7页World Sci-Tech R&D

基　　金：甘肃省自然科学基金项目(1506RJZA222);甘肃省高等学校科研项目(2014B-001)资助

摘　　要：目的建立小鼠创伤性脑损伤动物模型,进行神经干细胞移植,以评价神经干细胞对创伤性脑损伤的修复作用。方法体外培养神经干细胞并进行标记、鉴定,采用改良的Feeney氏自由落体法撞击构建创伤性脑损伤动物模型,脑外伤后1d进行神经干细胞移植,分别在移植当天(移植后0 d)、移植后3 d、7 d、14 d、21 d进行神经功能损伤程度(NSS)评分及疲劳转棒测试,移植后7 d、21 d进行抗Brdu、Nestin、GFAP、β-TubulinⅢ免疫荧光染色。结果移植后7 d、14 d、21 d神经干细胞移植组的NSS评分低于对照组,有统计学差异(P<0.05);移植后14 d、21 d神经干细胞移植组的疲劳转棒时间长于对照组,有统计学差异(P<0.05);移植后7 d免疫荧光染色发现BrdU/Nestin双阳性细胞,移植后21 d免疫荧光染色发现BrdU/GFAP双阳性细胞及BrdU/β-TubulinⅢ双阳性细胞。结论神经干细胞移植能够改善创伤性脑损伤后的神经功能,移植的神经干细胞能够在宿主脑内存活、迁移并在受损区域分化成星形胶质细胞和神经元。Objective The aim is to investigate the restoration function of neural stem cells （NSCs）transplantation in the treatment of traumatic brain injury （ TBI ）. Methods NSCs were cultured in vitro, labled with BrdU and identified before transplantation. The improved Feeney＇s method of free-fall impact was used to build traumatic brain injury model. One day later,NSCs was transplanted into the brain of TBI animal model. Neurological Severity Scores（NSS） and time of walking on the rotarod were used to test the function at the 0d ,3d,7d, 14d ,21 d post transplantation. Immun-ofluorescence staining anti BrdU, Nestin, GFAP, β-Tubulin m were carried on at 7d,21d post transplantation. Results There was significant difference in NSS between transplanting group and control group（ P 〈 0.05 ）at 7d, 14d,21 d post transplantation. There was significant difference in time of walking on the rotarod between transplanting group and control group（ P 〈 0.05 ）at 14d,21d post transplantation. The cells both BrdU and Nestin positive were found at 7d post transplantation. The cells both BrdU and GFAP positive were found at 21d post transplantation. The cells both BrdU and β-TubulinⅢpositive were found at 21d post transplantation. Conclusion NSCs transplantation is an effective method which can improve and protect neural function of TBI model. Transplanted NSCs can survive in host＇s brain,immigrate and differentiate into neurons and astrocytes in traumatic area.

关 键 词：神经干细胞 神经元 星形胶质细胞 神经球 创伤性脑损伤 分化 移植 

分 类 号：R651.15[医药卫生—外科学]