白族药紫金龙的HPLC指纹图谱研究  

HPLC Fingerprint of Dactylicapnos scandentis Radix by the Traditional Bai Medicine

作　　者：苏小军 杨怀镜 曾懿 闻 

机构地区：[1]云南省大理州食品药品检验所,云南大理671000

出　　处：《中国民族民间医药》2016年第4期1-3,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：中国食品药品检定研究院中青年发展研究基金(2013WA11)

摘　　要：目的：建立紫金龙药材HPLC指纹图谱分析方法,为紫金龙药材的质量评价提供依据。方法：采用RP-HPLC色谱法,Inertsil ODS-SP（4.6mm×150mm,5μm）色谱柱,以甲醇-0.2%磷酸水溶液（三乙胺调p H为7.0）为流动相梯度洗脱,柱温35℃,流速1.0ml/min,检测波长采用波长切换,0~40min为230nm,40~90min切换至285nm,进样量10μl;数据使用中药色谱指纹图谱相似度评价系统研究版（2012A）进行处理。结果：在选定的色谱条件下确定10个峰构成紫金龙药材的特征共有峰,各批次药材均具有上述特征,但特征峰的相对含量分布差异导致色谱概貌存在一定差异。结论：该方法简便、精密度高、重现性好,可为紫金龙药材的质量评价提供依据。Objective To establish the HPLC fingerprint of Dactylicapnos scandentis radix and to research the quality of Dactylicap- nos scandentis radix. Methods The gradient elution and multiple wavelength mode was applied in chromatographic separation, and chromatographic fingerprint similarity evaluation software （2012A） was used to analyse data. Results 10 peaks were identified as the characteristic fingerprints of Dactylicapnos scandentis radix. All samples tested contained the same 10 peaks, but the content of each peak showed great difference among the samples, which resulted in the differences on their chromatograms. Conclusion This method is simple, accurate with good reproducibility, and can be used for the quality control of Dactylicapnos scandentis radix.

关 键 词：白族药 紫金龙 特征图谱 HPLC 

分 类 号：R284.1[医药卫生—中药学]