滇黄芩总黄酮不同提取方法的比较分析  

Comparison and Analysis Different Extraction Methods of Total Flavonoids From Scutellaria amoena

作　　者：刘海鸥[1] 虎春艳[1] 普春霞[1] 刘晓玲[1] 林玉萍[1] 赵声兰[1] 

机构地区：[1]云南中医学院中药学院,昆明650500

出　　处：《中国民族民间医药》2016年第4期21-23,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：云南省教育厅重点项目(2014z119);云南省教育厅项目(2013y239)

摘　　要：目的:研究不同提取方法对滇黄芩总黄酮提取率的影响。方法:以滇黄芩总黄酮得率、干膏得率为指标,对煎煮法、乙醇回流提取法、纤维素酶-磷酸缓冲水溶液提取法、乙醇超声提取法、纤维素酶-乙醇超声提取法进行研究分析。结果:考察五种提取方法对考察指标的影响,计算综合评价指标Y值,Y值的大小顺序为纤维素酶-乙醇超声提取法>乙醇超声提取法>煎煮法>乙醇回流提取法>纤维素酶-磷酸缓冲水溶液提取法。结论:滇黄芩总黄酮的提取方法以纤维素酶-乙醇超声提取法为佳。Objective To research the extraction rate of different extraction of total flavonoids from Scutellaria amoena. Methods To optimize the extract technology of flovonoids for Scutellaria amoena, five methods such as water extraction, ethanol extraction, cel- lulose enzyme - phosphate buffer solution extraction, ethanol ultrasonic extraction method, cellulose enzyme - ethanol ultrasonic ex- traction method, were useed to extract flavonoids for Scutellaria amoena, also were compared and analyzed the flavonoids by adopting the total content of flavonoids and the yield of dry extract as markers. Results The comprehensive values Y ranked in the order of cellu- lose enzyme - ethanol ultrasonic extraction, ethanol ultrasonic extraction, water extraction, ethanol extraction, Cellulose enzyme - phosphate buffer solution extraction. Conclusion The optimal method to extract flavonoids for Scutellaria arrwena is Cellulose enzyme - ethanol ultrasonic extraction.

关 键 词：滇黄芩 总黄酮 提取方法 比较分析 

分 类 号：R284.2[医药卫生—中药学]