鹅细小病毒感染雏鹅血液的蛋白/酶活性及同工酶变异性研究  

Study on the Isozyme Variability and Activity of Protein/Enzymes from Gosling Blood Infected with Goose Parvovirus

作　　者：朱新产[1] 王倩文[1] 朱峰伟 李洪强[1] 

机构地区：[1]青岛农业大学生命科学学院,山东省高校植物生物技术重点实验室,青岛266109 [2]青岛即墨畜牧兽医局,即墨266200

出　　处：《中国畜牧兽医》2016年第2期413-422,共10页China Animal Husbandry & Veterinary Medicine

基　　金：青岛市科技发展计划(12-1-4-5-(2)-jch);山东省自然科学基金(ZR2011CM008)

摘　　要：为了解鹅细小病毒(goose parvovirus,GPV)侵染的病理学致病机理,探究蛋白/酶的分子变异特征,本研究对GPV感染雏鹅血液中的蛋白质、保护酶、蛋白酶活性及其同工酶结构和功能等进行了生化—分子生物学分析。结果显示,GPV感染雏鹅的血液中(血浆和血细胞)蛋白酶活性和蛋白质含量、过氧化物酶(POD)、超氧化物歧化酶(SOD)及酯酶(Est)活性分别是对照组的1.46和1.63、1.51和1.49、1.50和1.14、1.36和1.73、1.30和1.53倍。GPV感染雏鹅的血浆中,SOD同工酶增加了2条谱带(134和239);Est同工酶增加2条快区主带,减少了2条慢区谱带;酶蛋白减少1条慢区谱带(304);蛋白质新增加4条主带(131、86、43、33ku)。而血细胞新出现2条POD同工酶谱带(93和203),分别增加了1条(160)中区SOD同工酶谱带、1个快区Est同工酶谱带、2个慢区酶蛋白谱(223和330)和4个蛋白主带(144、104、58、53ku)。提示GPV感染应激与寄主基因表达的蛋白质、保护酶、蛋白酶及同工酶相互网络协作会引起酶的谱型、活性等生化特征变异,直接或间接调控代谢途径与病理生化性能,增强机体对入侵GPV的防御应激性能。因此,蛋白质、保护酶、蛋白酶及同工酶是GPV感染的应激靶标,可作为雏鹅易感GPV致病机制研究的有效标记。In order to understand the pathogenesis of GPV（goose parvovirus）,and explore the molecular variation of protein/enzyme,the protein,protective enzymes（Est,POD,SOD,protease）and isozyme from goslings blood infected with GPV were analyzed by biochemistry and molecular biology.The results showed that the activity of protease,content of protein,the activities of POD,SOD and Est（in plasma and blood corpuscle）from goslings infected with GPV were 1.46and1.63,1.51 and 1.49,1.50 and 1.14,1.36 and 1.73,1.30 and 1.53 times than that of control group,respectively.In the plasma of goslings infected with GPV,2bands（134and 239）of SOD isozyme appeared,2fast-zone enzyme band appeared and 2slow-zone enzyme band deleted in Est isozyme,1slow-zone zymoprotein band（304）deleted and 4new main protein bands（131,86,43 and 33ku）appeared.In the blood corpuscle of goslings infected with GPV,2new enzyme bands（93and 203）of POD isozyme,1medium-zone enzyme band（160）of SOD isozyme,1fast-zone en-zyme band of Est isozyme,2slow-zone zymoprotein band（223and 330）and 4new main protein bands（144,104,58 and 53ku）appeared.These results indicated that the interaction of GPV stress and host protein/enzymes and isozyme gene expression would result in the biochemical characteristics variation of activity and isozyme patterns of protein/enzymes,and directly or indirectly affect metabolic approach and pathological biochemical function on goslings infected with GPV,and enhance the defense and stress ability of GPV.Therefore,protein/enzymes and isozyme might be sensitive enzyme of GPV infection stress,and were effective marker of pathological mechanism on investigation of virus genotype interaction with genetic susceptibility of the host.

关 键 词：雏鹅 鹅细小病毒 蛋白/酶 保护酶 同工酶 

分 类 号：S858.33[农业科学—临床兽医学]