云南猪关节炎细菌性病原的分离鉴定  

Isolation and Identification of Bacterial Pathogen of Pig Arthritis in Yunnan

作　　者：周玉照 张小苗[1] 柴俊[1] 李三平[1] 张娜娜[1] 张以芳[1] 

机构地区：[1]云南农业大学动物科学技术学院

出　　处：《中国畜牧兽医》2016年第2期499-506,共8页China Animal Husbandry & Veterinary Medicine

基　　金：云南省重点新产品开发(2014BB04);云南省院士专家工作站建设项目(2014IC034)

摘　　要：本试验旨在了解云南4个猪场猪关节炎的细菌性病因及其耐药性。通过分离培养、生化试验、药敏试验、动物致病性试验及16SrRNA鉴定,对4个猪场出现猪关节炎症状的样品进行了分析。结果显示,A、B、C和D 4个猪场共96份样品中,检出金黄色葡萄球菌55份,阳性率分别为20.8%、83.3%、91.7%和33.3%;检出链球菌56份,阳性率分别为95.8%、21.7%、25.0%和83.3%;混合感染阳性率分别为20.8%、21.7%、25.0%和33.3%。因此,金黄色葡萄球菌是导致B、C两猪场猪关节炎的主要细菌性病原;链球菌是导致A、D两猪场猪关节炎的主要细菌性病原;两种细菌对菌必治、米诺环素和环丙沙星较敏感,对多种抗生素产生了耐药性。This experiment was conducted to investigate the bacterial causes and drug resistance of pig arthritis of four farms in Yunnan.Through isolation and culture,biochemical test,drug sensitive test,animal pathogenicity experiment and 16 SrRNA identification,we analyzed the samples from four farms with pig arthritis symptom.The results showed that in all 96 samples,55samples were Staphylococcus aureus positive,the positive rates were 20.8%,83.3%,91.7% and 33.3%in A,B,C and D farms,respectively;56samples were Streptococci positive,the positive rates were95.8%,21.7%,25.0% and 83.3%,respectively;Mixed infection rates were 20.8%,21.7%,25.0% and 33.3%,respectively.Therefore,Staphylococcus aureus was the major bacterial pathogen which caused pig arthritis in B and C farms;Streptococci was the major bacterial pathogen which caused pig arthritis in A and D farms;Two kinds of bacteria were more sensitive to ceftriaxone,minocycline and ciprofloxacin,and resistant to multiple antibiotics.

关 键 词：猪关节炎 金黄色葡萄球菌 链球菌 16S rRNA鉴定 

分 类 号：S858.61[农业科学—临床兽医学]