RH-HPLC法测定青蒿素中脱氢青蒿素及9-表-青蒿素的含量  

Determination of artemisitene and 9-epi-artemisinin in artemisinin raw material by RH-HPLC

作　　者：唐亮[1] 谭淑珍[1] 华坤鹏 王述维 

机构地区：[1]长沙理工大学化学与生物工程学院,湖南长沙410114 [2]湖南如虹制药有限公司,湖南岳阳414300

出　　处：《湖南文理学院学报（自然科学版）》2016年第1期24-28,34,共6页Journal of Hunan University of Arts and Science(Science and Technology)

基　　金：湖南省教育厅资助科研项目(14A012);湖南威嘉生物科技有限公司资助科研项目

摘　　要：建立了一种利用反相液相色谱法测定青蒿素原料药中脱氢青蒿素和9-表-青蒿素含量的分析方法。采用紫外检测器于210 nm处进行检测,以C-18柱（Agilent Zobax ODS,150 mm×4.6 mm,5μm）为分析柱,以乙腈和水的混合溶液为流动相,采取梯度洗脱方式（0～10 min,45%乙腈;10～20 min,45%～80%乙腈;20～25 min,80%～45%乙腈;25～35 min,45%乙腈）进行洗脱。在此条件下,青蒿素、脱氢青蒿素和9-表-青蒿素能彼此完全分离。脱氢青蒿素和9-表-青蒿素的浓度分别在4.11～28.76、9.42～65.93μg/m L范围内与峰面积的线性关系良好,相关系数R均大于0.998,检出限（3S/N）分别为2.668 ng和75.34 ng,样品加标回收率均在94%～108%之间。A reversed-phase high performance liquid chromatography （RP-HPLC） is developed for the determination of artemisitene and 9-epi-artemisinin in artemisinin raw material. The method employs a UV detector at 210 nm by using a C-18 column （Agilent Zobax ODS, 150 mm ～ 4.6 mm, 5 μm）. The mobile phase consists of acetonitrile and water with different volume ratios and is controlled by a gradient program, in which 45% acetonitrile is kept for 10 min, followed by a linear 10 min gradient to 80% acetonitrile, a linear 5 min gradient to 45% acetonitrile, and maintaining 45% acetonitrile for 10min to recondition the column. Under above-mentioned conditions, the peaks of artemisinin, artemisitene and 9-epi-artemisinin can be separated each other completely. There are good linear relationships between peak area and concentration in the range of 4.11～28.76μg/mL for artemistene, and in the range of 9.42～65.93 μg/mL for 9-epi-artemisinim, the correlation coefficients （R） are all more than 0.998. The limits of detection （（3S/N）） are 2.668 ng and 75.34 ng, respectively. The recoveries by standard addition method are all in the range of 94%～ 108%.

关 键 词：青蒿素 脱氢青蒿素 9-表-青蒿素 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]