汉黄芩素逆转卵巢癌SKOV3细胞对顺铂的耐药性  

Reversal effect of Wogonin on cisplatin-resistance of human ovarian cancer cells

作　　者：张娜[1] 周琦[2] 陆松梅[1] 吴顺龙[1] 刘双[1] 何燕琼[1] 李少林[1] 

机构地区：[1]重庆医科大学基础医学院放射与肿瘤教研室,重庆400016 [2]重庆市肿瘤医院妇瘤科,重庆400030

出　　处：《肿瘤》2016年第2期166-172,共7页Tumor

摘　　要：目的 :研究汉黄芩素是否可以逆转顺铂(cisplatin,DDP)耐药性卵巢癌SKOV3/DDP细胞对DDP的耐药性,并探讨其可能的分子机制。方法 :用终浓度为20μmol/L的汉黄芩素作用SKOV3/DDP细胞24 h,同时以SKOV3细胞和未经汉黄芩素作用的SKOV3/DDP细胞作为对照。然后,采用CCK-8法检测各组细胞的增殖能力,细胞划痕愈合实验和Transwell侵袭实验检测不同组细胞的侵袭和迁移能力,蛋白质印迹法检测不同组细胞中P-糖蛋白(P-glycoprotein,P-gp)、E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)和波形蛋白(vimentin)的表达水平。结果:汉黄芩素处理后,SKOV3/DDP细胞对DDP的敏感性明显提高(P<0.05),迁移能力和侵袭能力均明显降低(P值均<0.01),N-cadherin和vimentin表达水平均明显下调(P值均<0.01),而E-cadherin表达水平明显上调(P<0.01),P-gp水平则无明显变化(P>0.05)。结论:汉黄芩素能增加SKOV3/DDP细胞对DDP的敏感性,其作用机制可能与逆转细胞的上皮-间质转化有关。Objective： To investigate the reversal effect of Wogonin on cisplatin（DDP）-resistance of human ovarian cancer SKOV3/DDP cells, and to explore its possible mechanism.Methods： SKOV3/DDP cells were treated with 20 μmol/L Wogonin for 24 h, while the SKOV3 cells and the SKOV3/DDP cells without any treatment were used as the controls. The cell proliferation ability was detected by CCK-8 method. The cell migration and invasion abilities were detected by scratch wound healing and Transwell invasion assay, respectively. Furthermore, the expressions of P-glycoprotein（P-gp）, E-cadherin, N-cadherin and vimentin proteins were detected by Western blotting.Results： After treatment with Wogonin, the sensitivity of SKOV3/DDP cells to DDP was increased（P 0.05）, and the migration and invasion abilities were significantly decreased（both P 0.01）; the expressions of N-cadherin and vimentin proteins were downregulated（both P 0.01）, but the expression of E-cadherin was up-regulated（P 0.01）, while there was no significant difference in the expression of P-gp among different groups（P 0.05）.Conclusion： Wogonin may increase the sensitivity of SKOV3/DDP cells to DDP by reversing epithelial-mesenchymal transition.

关 键 词：卵巢肿瘤 抗药性 肿瘤 汉黄芩素 顺铂 上皮-间质转化 

分 类 号：R737.3[医药卫生—肿瘤]