不同加工方法何首乌中多元功效物质的测定及主成分分析  

Determination of multiple functional substances in different processed products of Polygoni Multiflori Radix and principal component analysis

作　　者：罗益远 刘娟秀[1] 刘训红[1] 兰才武 侯娅[1] 马阳[1] 王胜男[1] 蔡宝昌[1] 

机构地区：[1]南京中医药大学,江苏南京210023 [2]贵州昌吴中药发展有限公司,贵州凯里556000

出　　处：《中草药》2016年第2期318-323,共6页Chinese Traditional and Herbal Drugs

基　　金：国家科技支撑计划项目(2011BAI13B04);江苏高校优势学科建设工程资助项目(ysxk-2014);江苏省中药炮制重点实验室开放式课题(zypz007)

摘　　要：目的考察不同加工方法对何首乌中多元功效物质的影响及其变化规律,以优化及建立何首乌适宜的产地加工方法及其条件。方法采用胶束电动毛细管色谱二极管阵列检测法(MEKC-DAD)测定12种不同加工方法的何首乌药材中二苯乙烯苷、游离型蒽醌、结合型蒽醌及儿茶素;将多元功效物质分析结果标准化处理,进行主成分分析(PCA),对不同加工方法进行综合评价。结果不同加工方法的何首乌中多元功效物质的量变化呈现一定规律性,二苯乙烯苷的量及结合蒽醌的总量在厚片晒干中最高;游离蒽醌的总量在厚片70℃烘干中最高;儿茶素的量在薄片40℃烘干中较高;何首乌药材厚片晒干综合评价指数明显高于其他加工样品。结论加工方法对何首乌化学成分具有一定的影响,可根据目标成分优选何首乌适宜产地加工方法。Objective To study the effect of different processing methods and conditions for bioactive constituents in Polygoni Multiflori Radix（the root tubers of Polygonum multiflorum） and their change regularity, in order to optimize and establish appropriate drying methods and conditions. Methods The stilbene glucoside, free-anthraxquinones, combined-anthraxquinones, and catechin from 12 kinds of Polygoni Multiflori Radix in different processing methods were determined by MEKC-DAD. The multiple functional substances of principal component analysis（PCA） was made to standardized treatment for the comprehensive evaluation of the 12 samples by different drying methods. Results The contents of multiple functional substances in different processed products from Polygoni Multiflori Radix presented the certain regularity. The contents of stilbene glucoside and combined anthraquinone were the highest in Polygoni Multiflori Radix which was cut into thick pieces dried in the sun, the total free anthraquinones were the highest in Polygoni Multiflori Radix which was cut into thick pieces dried at 70 ℃, the content of catechin was the highest in Polygoni Multiflori Radix which was cut into thin pieces dried at 40 ℃. The comprehensive evaluation index obtained with PCA showed that the sample cut into thick pieces dried in the sun was significantly higher than those with other samples. Conclusion The influence of processing methods by the determination of multiple functional substances in Polygoni Multiflori Radix has been investigated and a scientific basis for the suitable processing method of Polygoni Multiflori Radix provided.

关 键 词：何首乌 胶束电动毛细管色谱二极管阵列检测法 多元功效物质 产地加工 二苯乙烯苷 

分 类 号：R282.4[医药卫生—中药学]