儿童感染的志贺菌Ⅰ类整合子表达及其与产超广谱β-内酰胺酶基因关系的研究  

Correlation with ESBLs Genes and ClassⅠIntegron of Shigella Spp. in Children

作　　者：蒋鸿超[1] 奎莉越[1] 黄海林[1] 苏敏[1] 樊茂[1] 王爱萍[1] 

机构地区：[1]昆明医科大学附属儿童医院,云南昆明650000

出　　处：《儿科药学杂志》2016年第2期1-5,共5页Journal of Pediatric Pharmacy

基　　金：昆明市卫生科技人才培养项目;编号SW(后备)-75

摘　　要：目的:研究儿童感染的志贺菌Ⅰ类整合子表达及其与产超广谱β-内酰胺酶(ESBLs)基因的相关性。方法:采用PCR方法检测60株产ESBLs志贺菌基因分型以及检测60株产ESBLs与60株非产ESBLs志贺菌Ⅰ类整合酶基因,以分析Ⅰ类整合子与细菌耐药性的关系以及与ESBLs基因的关系。结果:儿科临床产ESBLs志贺菌耐药基因分型以CTX-M型最多见(85.0%),其次为TEM-1型(50.0%)。产ESBL菌株基因分型分布情况以CTX-M型最多见(56.7%),其次为TEM-1+CTX-M型(20.0%)和TEM-1型(20.0%)。产ESBLs和非产ESBLs菌株中Ⅰ类整合酶扩增阳性例数分别为55例(91.7%)和15例(25.0%),两组整合子阳性率比较差异有统计学意义(P<0.01),同时Ⅰ类整合子表达与ESBLs具有相关性(R=0.67,P<0.01);Ⅰ类整合子阳性的菌株耐药性明显高于Ⅰ类整合子阴性的菌株,整合子阳性株对环丙沙星、左氧氟沙星以及复方磺胺甲唑耐药率较高,分别为84.3%、58.6%和90.0%。结论:儿童感染的产ESBLs志贺菌中Ⅰ类整合子携带率明显高于不产ESBLs菌株,显示儿童感染的志贺菌Ⅰ类整合子与ESBLs基因之间存在密切相关性。Objective： To study the correlation with ESBLs genes and classⅠintegron of Shigella spp. in children. Methods： PCR was used for detections of ESBLs genotyping in the 60 strains ESBLs-producing Shigella spp. and class Ⅰ integrase gene in the 60 strains ESBLs-producing Shigella spp. and 60 strains of non-ESBLs producing Shigella spp.. The relationship between class Ⅰ integron and drug resistance,the relationship between class Ⅰ integron and ESBLs genes were analyzed. Results： The most common genotype of ESBLsproducing Shigella spp. in pediatrics was CTX-M（ 85.0%）,followed by TEM-1（ 50.0%）. The predominant distribution of genotype in ESBLs-producing strains was CTX-M（ 56.7%）,followed by TEM-1＋CTX-M（ 20.0%） and TEM-1（ 20.0%）. Class Ⅰ integrase gene detected in ESBLs-producing and non-ESBLs-producing strain were 55 cases（ 91.7%） and 15 cases（ 25.0%） separately,the difference was statistically significant（ P〈 0. 01）. Expression of class Ⅰ integron highly correlated with ESBLs gene（ R = 0. 67,P〈 0. 01）. Drug resistance in class I integron positive strains were significantly higher than in class I integron negative strains,especially in ciprofloxacin,levofloxacin and sulfamethoxazole（ 84.3%,58.6% and 90.0%）. Conclusion： The distribution of classⅠintegron in ESBLs-producing Shigella spp.which children infected is significantly higher than that in non-ESBLs-producing strains,it is rational that classⅠintegron highly correlate with strong drug resistance in ESBLs-producing strains.

关 键 词：整合子 耐药基因 儿童 超广谱Β-内酰胺酶 

分 类 号：R378.99[医药卫生—病原生物学]