血清同型半胱氨酸及亚甲基四氢叶酸还原酶与先兆流产相关性研究  

Study on correlation between serum homocystine and methylenetetrahydrofolate reductase with threatened abortion

作　　者：叶贵诚[1] 王冬娥[1] 程立子[2] 袁春雷[1] 彭建明[1] 

机构地区：[1]广东省中山市博爱医院检验中心,528403 [2]广东省中山市博爱医院生殖中心,528403

出　　处：《检验医学与临床》2016年第4期443-445,共3页Laboratory Medicine and Clinic

基　　金：中山市科技计划资助项目(20102A131)

摘　　要：目的探讨血清同型半胱氨酸(Hcy)水平及亚甲基四氢叶酸还原酶基因(MTHFR)C677T、A1298C位点多态性与先兆流产的关系。方法以93例先兆流产患者为病例组,99例健康孕妇为对照组,采用荧光定量PCR技术检测MTHFR基因C677T和A1298C位点多态性,同时应用酶循环法测定血清Hcy水平。比较病例组和对照组间基因型分布频率和血清Hcy水平差异。结果病例组血清Hcy水平明显高于对照组;高Hcy是先兆流产的危险因素(OR=2.056,P=0.016);MTHFR C677TT基因型携带者血清Hcy水平明显高于其他基因型;A1298CC基因型携带者血清Hcy水平明显高于AA野合型;病例组A1298CC纯合基因频率明显高于对照组;差异均有统计学意义(P<0.05)。结论 Hcy升高是导致先兆流产的重要危险因素;MTHFR A1298CC纯合突变可致血清Hcy水平升高与先兆流产的发生有一定的相关性。Objective To investigate the relationship between homocystine（Hcy）and methylenetetrahydrofolate reductase（MTHFR）gene C677 T,A1298Csite polymorphism with threatened abortion.Methods 93 cases of threatened abortion and 99 healthy pregnant women were selected as the cases group and the control group respectively.The polymorphism of C677 T,A1298Cin MTHFR gene were detected by the fluorescence quantitative PCR,meanwhile the level of serum Hcy was tested by the enzymatic cycling assay.The differences in genotypes distribution frequency and serum Hcy level were compared between the two groups.Results The serum Hcy level in the case group was remarkably higher than that in the control group;the high Hcy level was the risk factor of threatened abortion（OR=2.056,P=0.016）;the serum Hcy level in the C677 TT genotype carriers was remarkably higher than that in the other genotypes carriers;the serum Hcy level in the A1298 CC genotype carriers was significantly higher than that in the AA genotype carriers;the frequency of MTHFR A1298 CC isozygoty gene in the case group was obviously higher than that in the control group,the differences were statistically significant（P〈0.05）.Conclusion The increase of serum Hcy level is an important risk factor of threatened abortion;the isozygoty mutation of MTHFR A1298 CC can cause the increase of serum Hcy level and has certain relation with the occurrence of threatened abortion.

关 键 词：同型半胱氨酸 亚甲基四氢叶酸还原酶 先兆流产 相关性 

分 类 号：R587.1[医药卫生—内分泌]