慢性丙型病毒性肝炎患者CD4^+CD25^+ Treg细胞与自身抗体的相关性研究  

The study on the correlation between CD4^+CD25^+ Treg cells and serum autoantibodies in patients with Chronic Hepatitis C

作　　者：卞成蓉[1] 刘佳[2] 陈霖[1] 刘爱霞[1] 宋英伟[3] 戴亮[1] 张岚[1] 李伯安[1] 

机构地区：[1]中国人民解放军第三○二医院临床检验医学中心,北京100039 [2]中国人民解放军第三○二医院输血科,北京100039 [3]中国人民解放军总医院输血中心,北京100853

出　　处：《检验医学与临床》2016年第4期451-453,456,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨慢性丙型病毒性肝炎患者CD4^+CD25^+Treg细胞水平与自身抗体之间的关系及其临床意义。方法选取2013年10月至2015年2月该院收治的324例新入院且未经治疗的慢性丙型病毒性肝炎患者,利用间接免疫荧光和免疫印迹方法检测患者自身抗体谱的表达状况,同时使用流式细胞术计数患者外周血CD4^+CD25^+Treg细胞数量。结果 324例慢性丙型病毒性肝炎患者,有136例患者至少有1项自身抗体阳性,另有188例慢性丙型病毒性肝炎患者自身抗体谱表达均为阴性。自身抗体阳性组CD4^+CD25^+Treg细胞百分比明显低于自身抗体阴性组(P<0.05);同时慢性丙型肝炎患者CD4^+CD25^+Treg细胞水平随着自身抗体表达数的增加而降低,呈负相关。结论对HCV感染患者进行CD4^+CD25^+Treg细胞水平监测,能有效预判患者发生免疫耐受的可能性。Objective To investigate the relationship and the clinical significance between CD4~＋CD25~＋Treg cells and autoantibody in patients with Chronic Hepatitis C.Methods Newly hospitalized and untreated 324 patients with chronic hepatitis C in our hospital were selected from October in 2013 to February in 2015.Using the methods of indirect immunofluorescence and Western blot,we observed the expression of serum autoantibody profiles in hepatitis C patients.Meanwhile,the amount of CD4~＋CD25~＋Treg cell in peripheral blood of patients with Chronic Hepatitis C were detected by flow cytometry.Results 136 patients with chronic hepatitis C in 324 cases had at least one positive autoantibody before treatment,none of other 188 patients had a positive autoantibody.The amount of CD4~＋CD25~＋Treg cell in peripheral blood of patients with Chronic Hepatitis C prompted that the percentage of CD4~＋CD25~＋Treg cells in the positive group was significantly lower than that in the negative group（P〈0.05）.What′s more,CD4~＋CD25~＋Treg cells of patients decreased with the increase of the positive number of autoantibody,which was negatively correlated with each other.Conclusion Monitoring the level of CD4~＋CD25~＋Treg cell in patients with HCV infection could effectively predict the possibility of immune tolerance.

关 键 词：慢性丙型肝炎 自身抗体 CD4^+CD25^+ TREG细胞 

分 类 号：R563.101[医药卫生—呼吸系统]