DNA甲基转移酶与甲基化CpG结合结构蛋白2在胃肠间质瘤的表达  

Expression of DNA methyltransferase and methyl-CpG-binding domain protein 2in gastrointestinal stromal tumor

作　　者：何苗[1] 范晶[2] 林劼 王子卫[4] 赵和照[1] 

机构地区：[1]重庆市肿瘤研究所胃肠外科,重庆400030 [2]重庆医科大学附属第一医院急诊科,重庆400016 [3]重庆医药(集团)股份有限公司,400011 [4]重庆医科大学附属第一医院普通外科,重庆400016

出　　处：《检验医学与临床》2016年第4期469-471,共3页Laboratory Medicine and Clinic

摘　　要：目的研究DNA甲基转移酶(DNMT)和甲基化CpG结合结构蛋白2(MBD2)在胃肠道间质瘤(GIST)的蛋白表达。方法采用免疫组织化学法与免疫印迹法对28例成人GIST标本及配对对照标本进行DNMTs与MBD2的蛋白表达研究。结果 DNMT1、DNMT2、DNMT3a、DNMT3L、MBD2蛋白在GIST中表达显著高于配对对照组织,差异有统计学意义(P<0.05),但DNMT3a在GIST的表达差异无统计学意义(P>0.05)。结论 GIST表达DNMTs(除外DNMT3a)和MBD2更显著。Objective To investigate the protein expressions of DNA methyltransferase（DNMT,including DNMT1,DNMT2,DNMT3 aa,DNMT3band DNMT3L）and methyl-CpG-binding domain protein 2（MBD2）in gastrointestinal stromal tumor（GIST）.Methods Immunohistochemistry and Western blotting were used to detect DNMT and MBD2 expressions in 28 pairs of adult GIST and matched non-tumor tissue.Results The protein expressions of DNMT1,DNMT2,DNMT3 b,DNMT3L and MBD2 were significantly stronger in adult GISTs than in matched non-tumor tissues（P〈0.05）;the protein expression of DNMT3 a,however,did not differ significantly between tumors and non-tumor tissues（P〉0.05）.Conclusion GISTs show protein expressions of DNMT（except DNMT3a）and MBD2 strongly.

关 键 词：DNA甲基转移酶 甲基化CpG结合结构蛋白2 胃肠道间质瘤 

分 类 号：R735.7[医药卫生—肿瘤]